大鼠視神經切斷后視網(wǎng)膜雙極細胞上PKC—alpha和recoverin的表達變化.pdf

1、中南大學碩士學位論文大鼠視神經切斷后視網(wǎng)膜雙極細胞上PKC—alpha和recoverin的表達變化姓名：蔡艷申請學位級別：碩士專業(yè)：神經生物學指導教師：羅學港20050501碩士學位論文摘要ChangesofexpressionofPKCalphaandrecoverinintheretinalbipolarcellsofadultratsaftertheopticnervetranseetedABSTRACTItiswellknow

2、nthatthereexistsantegradedegenerationafteropticnerveinjtuy[toweverrecentstudieshavedemonstratedthatinvisualnervesystem，inadditiontoantegradedegenerationtherealsoexiststransnearaldegenerationButtoourknowledgetheinformatio

3、naboutofretinalbipolarcellsisnotavailableuntillnowInthisstudywetestedthehypothesisthatretinalbipolarcellsareundergoingchangesafteropticnervetransectionFortyoneadultSDratswererandomlydividedinto3groups：normalcontrol(n=5)，

4、shamoperation(n26)andexperimentalgroup(opticnervetransected，n=30)Theanimalswereallowedtosurvivefor3，5，7，14，21and28daysthensacrifiedrespectivelynleretinaeswereremovedanddetectedbyimmunohistochemistryandimmunofluorescenceu

5、singspecificantibodiesagainstPKCalphaandrecoverinWefoundthat①InnormalretinaPKCalphapositiveproductsweremainlylocalizedintheinnernuclearlayer(INL)，innerplexiformlayer(IPL)andganglioncelllayer(GCL)，especiallyintheIPL，Axons

6、andexpandedterminalsofbipolarcellswereclearlyshown②Comparedwiththenormalcontrol，thenumberofpositiveterminalbuttonsintheIPLwasgraduallyincreasedfrom3days，7daysto14days，followedbyadecreaseatthe21stday③Innormalretinarecover

7、inpositiveproductsweremainlylocalizedinouternuclearlayer(ONL)，INLandGCLIntheasublayerandbsub—layerofIPL，axonsofbipolarceils’expandedterminalswereclearlystainedRecoverinpositiveproductswereshownclearlyastwoparallelbandsAf

8、teropticnervetransection(ONl)，thenumberofrecoverinpositivebipolarceilsgraduallydecreased，incontrastthattheirterminalbuRonsgraduallyincreasedandreachedtopeakatday14，thendroppedatday21and28withaLocaldisarrangementWeconclud

9、edthatthisdatamightsuggestthatafteropticnervetransection，retinalbipolarcells，amaerinecellsandRGCsareundergoingchangesofplasticitybomstructurallyandfunctionallyKeywords：opticnervetransection，PKC—alpha，recoverin，terminalbu