蘋果黑星病菌遺傳多樣性的SSR分析.pdf

1、西北農林科技大學碩士學位論文蘋果黑星病菌遺傳多樣性的SSR分析姓名：董艷玲申請學位級別：碩士專業(yè)：植物病理學指導教師：楊家榮20050601GENETICDIVERSITYOFVenturiainaequalisOFAPPLEWITHSSRANALYSISABSTRACTApplescab，causedbyVenturiainaequalis(Cooke)Wint，isoneofthemostseriousdiseasesofapple

2、andornamentalcrabappleBothleavesandfruitsCallbeinfectedInfectedleavesmaydropearlyresultinginweakeningtrees，withpoorfruitproductionInfectedfruitsareblemishedandoftenseverelydeformedInChinaapplescab，whichlimitistoonlyOccur

3、inafewlocalsites，ispointedasquarantineobjective，butinrecentyears，thisdiseaseisexpandingtOlargerareasandseverelythreateningtoappleindustryinChinaItwasanewdiseasefoundedfirstlyin1997inXinping，Liquan，Yangling，andXunyiinShaa

4、nxiprovinceThemainresultsobtainedbymolecularanalysiswithSSRthchniqueareasfollows：1ThegenomicDNAofVenturiainaequalisofapplewasextractedbyuseingSquashBlotmethod，whichcarlreducetheharmoftoxicitytoexperimenters，chemicalreage

5、ntsandneedinexpensiveapparatus，ThemainadvantageofthistechniquecangreatlyspeedupexperimentandissimpleandeasytooperationManysamplescanbecrushedontoasinglemembranepiece，andasingleoperatorCananalysehundredsofsamplesinonedayI

6、tcangreatlycutshortofthetimeofextractinggenomicDNAfromVenturiainaequalisofappleUsuallytheconventionalmethodtookmorethanonemonthtoextractgenomicDNAfrommyceliumgrowninculturemedium，butbyusingthismethodonlytookseveraldays2W

7、ehavesetuptheoptimizingSSRreactionsystemofVenturiainaequalis：each25ItLamplificationreactionsolutionWascomprised10xBuffer(MgCl220mmol／L)25ItL，dNTPl00gmoL／L，primers05lamol／L，TaqDNApolymerase15U，templateDNA2mg／L，ddH20195雎L。

8、Amplicationprogramis：2minat93(2，30Sat57“(2：then40cyclesthatconsistedof1minat72℃。30Sat93℃，30Sat57℃；followedby10minextensionat72℃TheresultofPCRamplificationshowedthatthePCRsystemhadgoodrepeatandstability3Wehaveanalyzedtheg

9、eneticdiversityof160isolatesoriginedfromUK，IndiaandSharmxiprovinceofChinaResultsshowedthatisolatesodginedfromChinaUKandmostofisolatesoriginedfromIndiahadcloserelationship，butsomeIndia’SisolatesRDl，G1—10，RI1，G28，G12andRI1

10、2variedinsomedgree，andtherewerenorelationship、vi也thosefromShannxiprovinceofChinaandUKResultsalsoindicaredthatthereWasnelatetionshipbetweenthegeographicdistanceanddiffemetapplecultivarsThisstudyearlhelpUStohavebeRerunders