三角紫葉酢漿草組培及碳源效應(yīng)的研究.pdf

1、Y783735分類號tS68236Q81312四川農(nóng)業(yè)大學(xué)碩i學(xué)位論文三角紫葉酢漿草組培及碳源效應(yīng)的研究王利指導(dǎo)教師：王丞渣數(shù)授學(xué)科專業(yè)：國掛撞物生理送國莖研究方向：國撻撞塹越壁差塑塑鳘堡中國四川雅安二oo五年五月StudyonTechniqueSystemofTissueCultureandEffectsofDifferentCarbonSourcesinOxalistriangularisSubegTriangWangLi(Coll

2、egeofForestryandHorticulture，SichuanA班culturalUniversityYa’an625014，SiChuan，China)AbstractTheleavesandleafstalkswereusedasexplantstoestablishthetechniquesystemoftissuecultureandtostudytheeffectsofdifferentcarbonsourcesin

3、OxalistriangularisSubegTriangWithorthogonaldesign，anal)sisofvarianceandmultiplecomparisons，thebestmediumcomposmousandthecultureprocesswereaualyzedBesides，theeffectsofsucrose，glucoseandsolublestarchOilplantregeneration，co

4、lourandfloweringwerealsoinvestigatedTheresultswereasfollows：(1)MS6BA05mg幾十NAA05mg／LIBA01medLgaverisetothegoodresultininducingadventitiousbudsfromleafexplants，inwhichtheinducementratecouldbe90％(2)MSKT10mg／LNAA05mg／LIBA05m

5、VLWaSthebestmediumininducingadventitiousbudsfromleafstalkexplantswheretheinducementratecouldbe93j3％(3)MSsupplementedwith6BAl5mg，Land02mg，LNAAwasfavorabletotheproliferationofbuds，inwhichthemultiplicationratecouldbe444(4)T

6、hegrowthofrantswasbeston1／2MSmediumaddedNAA02mg／L，Wheretherootingratecouldbe95％(5)SucroseWaSthebestcarbonSOurCeinregeneratingplantletsfromleafexplants3％Wasthebestsucroseconcentrationinwhichtheplantregenerationratewashigh