美味牛肝菌多糖高產(chǎn)菌株的選育及其發(fā)酵工藝研究

1、湖北工業(yè)大學(xué)碩士學(xué)位論文美味牛肝菌多糖高產(chǎn)菌株的選育及其發(fā)酵工藝研究姓名：陽飛申請學(xué)位級別：碩士專業(yè)：發(fā)酵工程指導(dǎo)教師：張華山20090501湖北工業(yè)大學(xué)碩士學(xué)位論文AbstractBoletusedul西isectotrophicmycorrhizafungi，whosepolysaccharideshassignificanteffectontumourScreeningofhigllyieldpolysaccharidesstra

2、instreatedboletusedul西wasdeterminedinthisstudyusingprotoplastcompoundmutagenesisofUltravioletLiCl(uvLiCl)atthefirsttimeandwealSOstudyonthefermentationprocessofthemutantstrainthatwasnamedUL7Themainresearchresultsareausfol

3、lows：1、InordertoimprovethegrowthofBoletuseduliswhosemotherculturewehadscreened，wealSOoptimizedtheprocessofpolysaccharidesextractionandmadesurethemethodmeasuredpolysaccharidesbyexpefientBystudyingoncompoundprotoplastmutag

4、enesisofU、，LiClofthemotherstrain(ACCC50559)wecandrawaconclusionthatthebestconditionsoftheprotoplastcompoundmutagenesisofUVLiClThebestconditionsforprotoplastpreparationareasfollows：themyceliaincubatedfor52hweremostsuitabl

5、eforprotoplastreleasewiththemixturesolutionof0029／mLcellulaseand0029／mLsnailase(volumeratiol：1)，06m01／LMannitolaSthemosteffectiveosmoticstabilizer，thesuitableenzymetemperatureandtimeforthereleaseofprotoplastwasfrom25to29

6、℃and2hrespectivelyWiththeoptimumconditionsthenumberofprotoplastandtheregenerationratewasupto350100／mLand714％respectively；InduceddoseofcompoundprotoplastmutagenesisofUvLiClareasfollowing：30cmfrom15Wultraviolet1ightprotopl

7、astwasirradatedfor6minandspreadedontheplateofLiClwhoseconcentrationwas004％culturedfor10daysinshade，deathratewasupto796％Carryingoutthetrainingofmutantstrainsontheplatecultureandfermentationtests，throughthepreliminarysecti

8、onandthecontinualselecction，wefinallyacquiredonegeneticstabilityandhi曲一yieldofpolysaccharidemutantstrainthatwasnamedUL7，whosecolonydensityandmyceliagrowthwasmorethickeranddenser，grewfasterwithactivityofproducingpolysacch

9、arideswas39729L～419％higherthantheoriginalone；2、nlrou曲studyingonpolysaccharidefermentationprocessofthemutantstrainofUL7wecanconcludtheoptimizationexpefimentalconditionFormulaoftheoptimumliquidfermentationpolysaccharidemed

10、iumwasasfollows(％)：sugar2，peptone06，ZnS047H20010，CaC0301，KH2P0403，MgS047H2002，potatoes10；Liquidfermentationpolysaccharideoptimumfermentationconditionsareasfollowing：initialpHvaluewasfrom50to54oscillationspeedwas120r／rnin

11、，temperatureWasfrom25℃to28“C，capacityloadingwas40％(v／v)，plusing10glassbeads，fermentationtimewas5days，productionofpolysaccharidewasupto4361g／L：3、Onthebaseofstudyofp01ysacchandesfermentationprocessofthemutantstrainofUL7，at