玉米紋枯病抗性遺傳分析.pdf

1、四川農(nóng)業(yè)大學(xué)碩士學(xué)位論文玉米紋枯病抗性遺傳分析姓名：唐海濤申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：作物遺傳育種指導(dǎo)教師：榮廷昭楊俊品20040601AbstractThemaizeinbredline478whichissusceptibletomaizesheathblight、maizeinbredlineCML270whichisresistancetomaizesheathblight、three(CML270478)F2populations

2、、(CML270478)CML270backcmsspopulationand(CML270478)x478backcrosspopulationwereplantedThenthematerialswereinoculatedartificiallywithmaizesheathblightpathogenAG一1IATheresistancestatisticsshowedthattheresistancetomaizesheath

3、blightbehavesascharacterbetweenqualitycharacterandquantitycharacterTheheredityofresistancetomaizesheathblightismasterofatleastfourtosevenpairscentralgenes，whileitisinfluencedbymulti—geneswhichhavelowinfluencetotheheredit

4、yThegenomeDNAofmaizeinbredCML270and478wereextractedandproducedbyPCRwiththeprimersdesignedaccordingtothesequencesofknownplantdiseaseresistancegenesandtheconservedregionofthesegenesThesequencesofproductshadnohomologoustokn

5、ownplantdiseaseresistancegenes，buttheywerehomologoustootherknownplantgenesThesequenceoftheproductproducedbyPCRofthemaizeinbred478withtheprimerdesignedaccordingtothesequenceofPtogeneintomatowerehomologoustomaizeretrotrans

6、posongeneCinful一1WeestimatethesegmentispossiblypartofmaizeretrotransposongoneCinful一1TotalRNAin1eafsheathofmaizeinbredCML270wereextractedat0、6、12、24、36、48hrsafterbeinginoculatedartificiallywithmaizesheathblightpathogenAG

7、1一IATheeDNAretrotranscribedfromtheseRNAWereproducedbyPCRwiththeprimersdesignedaccordingtothesequencesofknownplantdiseaseresistancegenesandtheconservedregionofthesegenesTherewerenodifferencesbetweentheproductsofdifferentt

8、imeafterbeinginoculated塒tIlmaizesheathblightpathogenSoweestimatethattheexpressionofthegenesresistancetomaizesheathblightisconstitutivegenesKeywords：Zeamays，sheathblight，goneofresistancetodisease，heredityofresistancetodis