24440.蠟狀芽孢桿菌與重金屬鉻相互作用機制研究

1、福建農(nóng)林大學碩士學位論文蠟狀芽孢桿菌與重金屬鉻相互作用機制研究姓名：陳志申請學位級別：碩士專業(yè)：農(nóng)藥學指導教師：黃志鵬林璋20100401福建農(nóng)林：大學碩士學位論文AbstractAcomprehensiveunderstandingofthedetailedmechanismisexpectedtohaveasignificantimpactinthedesignoflow—costworkablestrategiesforthetr

2、eatmentandrecyclingofCr(VI)industrialwastesThoughtheinteractionmechanismbetweenCr(VDreducingbacteriaandCr(VI)wasextensivelystudied，howeverforaspecificbacteria，literaturesonlycontainoneortwopointsThecomprehensiveunderstan

3、dingoft11eCr(VI)uptakemechanismofaspecificbacterialstrainisquiteabsentInthisworkwithaCr(VI)tolerantstrainBacillusceYeus∞cereus)asanexample，completeunderstandingofCr(V1)reductionandCr(III)immobilizationmechanismwereolbtai

4、nedForthefirsttime，itWasfoundthattheextracellularsecretionofbacteriacouldplayallimportantroleduringtheCr(VI)reductionandCr(III)immobilizationprocessesProteindenaturantexperimentssuggestednoreducingenzymeparticipatedinthe

5、reductionprocessofCr(VI)bybothextracellularsecretionandcelldebrisofBcereusXrayphotoelectronspectroscopy(xPS)andelectronspinresonancespectroscopy(ESR)analysisrevealedthattheendproductwasCr(IlI)，withCr(V)asintermediateBoth

6、intracellularandextracellularimmobilizationofCr(III)wereobservedbyatomicforcemicroscopy(AFM)，scanningelectronmicroscopy(SEM)andtransmissionelectronmicroscopy(TEM)Inductivelycoupledplasmaopticalemissionspectroscopy(ICP—OE

7、S)analysisrevealedthattheCr(III)dis：tributionintracellularlyandextracellularlyWasabout314％and686％，respectivelyWithFouriertransforminfrared(FTIR)andextendedXrayabsorptionfinestructure(EXaFS)analysis，weconfirmedtheaminoand

8、carboxylgroupsfromthebacterialsurfaceaswellastheextracellularsecretionmightinvolveintheCrimmobilizationprocess，thepossiblecoordinationstructurecouldbesimilartoCr(Glycine)3H20Keywords：Bacilluscereus，chromium，reduction，imm