28084.兩種藥用菊科植物的化學(xué)成分及其抑制人乳腺癌細(xì)胞增殖作用的研究

1、華東師范大學(xué)碩士學(xué)位論文兩種藥用菊科植物的化學(xué)成分及其抑制人乳腺癌細(xì)胞增殖作用的研究姓名：李序文申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：基因組學(xué)指導(dǎo)教師：胡金鋒20100501華東師范人學(xué)碩上學(xué)位論文ABSTRACT1’womedicinalplants(Compositae)，鐋“砸“，e口cD伽f誼(Hemsl)andC口伊酷也肌扣6e廠f(winkl)havebeenchemicallyandbiolo舀callyinVestigatedfort

2、hefirsttimeNinenewandfony—sixknowncompoundswereisolatedbyusingcolumnchromato伊aphy(CC)oversilicagel，RP—C18，MCIgel，SephadexLH一20，PTLCandsemipreparatiVeHPLCTheirstmctures，especiallythestereochemistryofthenewcompounds，werech

3、aracterizedonthebasisofextensiVespectroscopicmethods(IR，UV1D一／2D—NMR，LR／HRESIMS，CifclJlarDichroism)柚dsomechemicaltransfbnnatiOnsniny—sixnaturallyoccurringC0mpounds，includingfiVeClo—acetylenic酉yCosides(1_4，6)，oneli印強(qiáng)(5)，s

4、ixphenylpropanoids(7_12)，ei曲tmegastigmenes(13—20)，twosesquite巾enes(2l，22)，fourmonoterpenes(23_26)，twocoumarins(27，28)，t王lreesterols(29_31)，onetrite叩ene(32)andafewmiscellaneousconstituents(3336)，wereisolatedfromthewholepl

5、antof勛“船“肥口cD以和婦Cbmpoundsl—5wereelucidatedasnewnaturalproductsSelectedcompoundswerecvaluatedfortheircytotoxicityagainsthum鋤breastcancer(MCF一7)cellsusingtheMTTassay，鋤d(8Ddecaene4，6diyn1一D嵋L廣rh鋤nopyr卸osyl(1—6)妒D酉ucopyranos

6、ide(3)and5口，夠dihydroxystigmastane3D事D一百ucopyranoside(31)werefoundtoshowmoderatecytotoxice骶cts，withIC50Valuesof297and230∥∥mLrespectiVelyNineteensecondarymetabolites，includingseVenteensesquite叩enelactones(37—53)andtwodite印

7、enoids(54，55)，wereisolatedf如mthewholeplantofC口印甜fH朋丘6e盹Compounds37—40weredeteminedt0benewstuctures舢lisolatedcompounds(3755)weretestedfortheircytotoxicityagainsthumanbreastc鋤cer(MCF7)ccllsusingtheMTTassay，鋤d2，3dihydroarom