30231.穿龍薯蕷微塊莖的低成本離體快繁及林下栽培的研究

1、東北師范大學(xué)碩士學(xué)位論文穿龍薯蕷微塊莖的低成本離體快繁及林下栽培的研究姓名：王花琴申請學(xué)位級別：碩士專業(yè)：遺傳學(xué)指導(dǎo)教師：王麗20090601AbstractDioscoreanipponica，whichisakindofimportantdiosgeninplantinChinaundernationalprotectionofsecondclass，誦thllighmedicalandeconomicvalueOwingtothe

2、uncontrolledexcavationinrecentyearsanddefectionoftraditionalcroppingpatterns，tissueculturehasamostlyexpansiveprospectonrapidpropagationofDioscoreanipponicaCultivationexpansioninwoodsisinaccordancewithcurrentecologicalnee

3、dsThestudyisofgreatsignificanceonboththeconservationofbiologicaldiversityandtomeetthemarketdemand。ByusiIlgnodalsegmentsofDioscoreanipponica謝thaxillarybudasexplants，weestablishedamicromberinductionsysteminvitroofDioscorea

4、nipponicaandmadeaoptimizationcontroltothegenerationofbasalmicrotuberandaerialmicrotuberFurthermore，thecostoftissuecultureWascutdownwimtheestablishmentofinductionsysteminvitroPreliminarystudyofitstubercultivationinthewood

5、swascarriedoutmeanwhileResultsaleasfollows：11MicrotuberinductionofDioscoreanipponicaMicrouberofDioscoreanipponicaCanbeobtainedwhenusing6benzylaminopurineandnaphthylaceticacidrespectivelyBycontrast，theinductionrateofbasal

6、micromberhasagreatenhancementwhenuseboth6benzylaminopurineandnaphthylaceticacid，aerialmicrotubercanbeobtainedatthesametimeThepropagationrateofeustipesWashighestwith6benzylaminopurine10mg／Landnaphthylaceticacid0Smg／LThein

7、ductionrateofbasalmicrotuberreached565％andaerialmicrotuber2826％whenusing6BA20megLandNAA05mg／LThehighconcentrationofsucroseinmediumwithouthormonesisfavourabletotheaerialmicromberabovethemediumandunfavourabletothebasalmicr

8、otuberunderthesurfaceofthemediumTheinductionrateofaerialmicrotuberatalllevelsishigherwhenusingsucrose，6BAandNAAsimultaneouslythanrespectivelyTheinductionratereached839％whenthesucroseconcentrationis309rL，SOCanbeusedasasui

9、tableaerialmicrotuberinductionmediumPhotoperiodichasnosignificanteffectoninductionofmicrotuber2)Cost—effectiveinvitropropagationmethodsforDioscoreanipponicaThecompositionofthemediumhasbeensimplifiedbyusingsoftsugarinstea