三種滲透性抗凍劑和稀釋基礎(chǔ)液配合對(duì)兔精液冷凍保存效果的研究.pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文三種滲透性抗凍劑和稀釋基礎(chǔ)液配合對(duì)兔精液冷凍保存效果的研究姓名：李孝娟申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：動(dòng)物繁殖學(xué)指導(dǎo)教師：張貴學(xué)2000.6.1棗章娟摘簧隸jb農(nóng)業(yè)大學(xué)CryoprotectiveeffectofthreepenetratingcryoprotectantcombinedwiththreebasicdilutionsonrabbitsemenAbstractGlycerol，dimethylsuffixe

2、d，acetamidewereadoptedtoverifythecryopfolectiveabilityofpentratingeryOprotect菇ntwiththreekindsofextendersSpermmobillty。Intactacrosomeratlo、Plasmamembraneintegrity(hypoosmoticswellingtest，HOST)wereassessedin矗esh，cooledfro

3、zenthawedsamPleandultrastructuralobservationwascarriedouttoanalyzetheeffectoffrozed—thawedThreeextendersareasfoowedf1)3，52％tris，196強(qiáng)citricacid，159％glucose；(2)3。03％tris，168％cJlrlcacid，l，25％glucose：(3)。5％sucrose，5％lactose，

4、andthreecryoprotectants：glycerolandDMSOendeoncentration(2％，3％，4％)andacetamideCOIlcentratlon(2％，4％，5囅)l：ldiluentioa，equilbrationat4℃for3hours，freezngat一90一95℃for10minutesandthawingat42℃for20secoadsTheresuitswereasfol／owed

5、：l：Theinfluenceonfrozen—lhawedspermmobltvlsdifferentwhelldlfferenteextenderiscomblnedwithd；fferentcryopf母￡eet鑫玨tThepertenrageofmotilespermishigherin4％glycerolAand5％acetamideC(P0。05)2：TheinfluenceonIntaet—acrosomesdiffere

6、ntwhendifferentexterideriscombinedwithdi糟霉rentcrvoDrotectantandacetamidehadbettuerprotectlveeffectthantheothersindesgnedconcerttrationsfP005)WithBdiluent，3％glycerolhadbettercfyopoteolVeabilityonmembranefunctionalintegrit