抗疏健骨顆粒對去勢大鼠骨、腎、下丘腦組織PPARγ2蛋白表達(dá)的影響.pdf

1、陜西中醫(yī)學(xué)院研究生學(xué)位論文獨(dú)創(chuàng)性與真實(shí)性聲明本人鄭重聲明：在我呈交的學(xué)位論文中，本人經(jīng)研究所得的數(shù)據(jù)、原理、結(jié)論等一切內(nèi)容均真實(shí)，且經(jīng)本人認(rèn)真核對無誤：我呈交的與學(xué)位論文相關(guān)的資料(原始記錄、照片、錄像片、檢查化驗(yàn)報(bào)告單等)確為完整的學(xué)位論文研究中的原始資料，本人沒作任何修改：我呈交的學(xué)位論文是我本人在指導(dǎo)教師的指導(dǎo)下進(jìn)行的研究工作及取得的研九成果。除文中特別加以標(biāo)注和致謝的地方外，論文中沒有其他人已經(jīng)發(fā)表或撰寫過的研究成果，其他人對我

2、完成此論文的幫助以及對本研究所做的任何貢獻(xiàn)均已在論文中作了明確說明并表示謝意。我呈交的學(xué)位論文及與該論文相關(guān)的資料若有不實(shí)之處，本人承擔(dān)一切相關(guān)責(zé)任。學(xué)位論文作者簽名：日期：2014關(guān)于學(xué)位論文使用授權(quán)的聲明本人完全了解陜西中醫(yī)學(xué)院有關(guān)保留使用學(xué)位論文的規(guī)定，同意學(xué)校保留或向國家有關(guān)部門機(jī)構(gòu)送交論文的印刷版和電子版，允許被查閱和借閱。本人授權(quán)陜西中醫(yī)學(xué)院可以將本學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫進(jìn)行檢索，可以采用影印、縮印或其他復(fù)印

3、手段保存和匯編本學(xué)位論文。(保密論文在解密后應(yīng)遵守此規(guī)定)學(xué)位論文作者簽名辨位論文導(dǎo)師簽名：’日期：2014年5月浮似望AbstractPurpose：ThroughtheexperimentsinfluenceofKangshujiangugranuleonovariectomizedratsbone，kidney，hypothalamictissueperoxisomepr01iferatoractivatedreceptorgam

4、ma2proteinexpression，fromthemolecularlevelresearchthemechanismofKangshujiangugranuleeffectsonosteoporosisinpostmenopausal，forKangshujiangugranuleareusedonPostmenopausalosteoporosisprovideexperimentalevidenceofclinicaltre

5、atmentMethod：Accordingtotheweightofeach1ayerandlayer，methodofrandomization，50femaleratsweredividedintofivegroups，respectively，intoshamoperationgroup，modelgroup，osteoporosisgroup，largedoseofKangshujiangugranulemediumdoseg

6、roup，10wdoseofKangshujiangugranulegroupUseofpostmenopausalosteoporosisinratmodelof，，classicmodeofovariectomizedmodel，，，themanufacturingofpostmenopausalosteoporosis：Kangshujiangugranulehigh—dosegroup，osteoporosisgroup，mid

7、dledoseofKangshujiangugranuleinsmalldosegroupandmodelgroupratswerebilaterallyovariectomizedmodelmanufacturingmenopauseosteoporosisShamoperationgroup，ovariectomizedaroundthesamesizethegreateromentum，fordifferencesbetweent

8、hegroupsfromoperationtrauma0neweekafterestablishingthemodel，intragastricadministrationKangshujiangugranuleofhighdosegroupwasgiven2869／kgofKangshujiangugranule，osteoporosisinmiddledosegroupweregiven1439／kgofKangshujiangug

9、ranule，Kangshujiangugranule10w—dosegroupweregiven0719／kgofKangshujiangugranule，adddistilledwaterusewillbeKangshujiangugranulepreparedintodifferentdrugsconcentrationsuspensiongavage：shamoperationgroupandmodelgroupweregive

10、n2mlnormalsalineAfter12weeksTaketheleftfemuron1／3，theleftkidney，1／2hypothalamictissueAfterdrawnweresacrificedDetectbone，kidney，relativecontentofhypothalamictissuePPARY2proteinexpressionusingWesternblotratsTheresultsanaly

11、zedbySPSS17OsoftwareResuIt：Bonetissueinmodelgroup：grayvalueofgrayvalueincreasedthanshamoperationgroup(P005)wasstatisticallysignificant，Kangshujiangugranuleinthetreatmentgroupthanthoseinthemodelgroupandgrayvalueofgrayvalu

12、edecreased(P005)wasstatisticallysignificant，comparisonbetweeneachdosegroup，middledosegroupwasthelowest(P005)graySignificance：inrenaltissueofmodelgroup：grayvalueofgrayvalueincreasedthanshamoperationgroup(P005)wasstatistic

13、allysignificant，Kangshujiangugranuleinthetreatmentgroupthanthoseinthemodelgroupandgrayvalueofgrayvaluedecreased(P005)wasstatisticallysignificant，comparisonbetweeneachdosegroup，middledosegroupwasthelowest(P005)graytherewa