四種禽類Sox8基因部分cDNA序列的克隆及其PCR-SSCP分析.pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文四種禽類Sox8基因部分cDNA序列的克隆及其PCRSSCP分析姓名：夏彥玲申請學(xué)位級別：碩士專業(yè)：動物遺傳育種與繁殖指導(dǎo)教師：劉娣2003.6.1CloningofFourFowls’Sox8GenePartialeDNASequenceAndPolymorphismAnalysisbyPCRSSCPAbstractTheSoxgonefamilyconstitutesagroupoftranscription

2、factorsAllfamilymembersarecharacterizedbypossessionofaspecifictypeofDNA—bindingdomainHMGboxSoxproteinshaveimportantfunctionsbothduringdevelopmentandinadultOneofthewellcharacterizedgroupsofSoxproteinsissubgroupESox8goneis

3、amemberofthesubgroopSox8gone，whichcontainsthreeexonsandtwointrons，isarecentlyidentifiedgene(Sehepers，GE，ota12000)MutatedSox8genewillresultinsomedevelopmentaldefectsThedeficientmiceofSox8gonewereviableandfertileBmasubstan

4、tialreductioninweightwasobservedinthesemice，especiallyinadiposetissueItsreductionislikelyduetochangesinenergyhomeostasisanditsregulation，suchasreducedefficacyoffoodutilizationanddisproportionateenergyallocationSothestudy

5、onitwillhavetheoreticalandpracticalsignificanceinanimalbreedingInthestudySox8paraaleDNAsequencesoffourspeciesWerCclonedbyRTPCRandcomparedthenueleotideandaminoacidsequenceshomologyofseveralspeciesTheresultshowsthatnucleot

6、ideandaminoacidsequencessurpasseidentityof96％and97％，respectivelyHomologyanalysisshows：Sox8goneishighconservationinfowlsInadditionthesevenpairsofprimersforSox8gonewefedesignedbasingontheeDNAsequenceofchikenandappliedtoana

7、lyzethepolymorphismofchickenSox8eDNAamongdifferentbreedsbyPCRSSCPTwoSNPswereidentifiedinthechickenSox8geneandsequencedTheresultshowsthatthereamtwomutationsinexon3(780C—T和1350G—A)butthetwomutantsitesdidn’tresultinchangeof

8、aminoacidStatisticalanalysisindicatestIlatfrequencyofaIleleAishigIlerinbroilersthalllayerSThereisatrendthatdistributionofalleleAispositivelyfelativetofatcharacterMasterCandidate：XiaYanlingSpeciality：Animalgeneticsandbree