東北地區(qū)水曲柳地理種源遺傳多樣性分析及優(yōu)良種源選擇.pdf

1、學(xué)校代碼：10225學(xué)號：05268學(xué)位論文東北地區(qū)水曲柳地理種源遺傳多樣性分析及優(yōu)良種源選擇指導(dǎo)教師姓名：申請學(xué)位級別：論文提交日期：授予學(xué)位單位：謝運海夏德安副教授東北林業(yè)大學(xué)碩士學(xué)科專業(yè)：林木遺傳育種2005年5月論文答辯日期：2005年6月東北林業(yè)大學(xué)授予學(xué)位日期：2005年6月答辯委員會主席：周顯昌研究員論文評閱人：張含國教授孫寶|)9Ⅱ研究員擎；剡霧素楚艨鑲，p】≯春東北林業(yè)大學(xué)碗士學(xué)位論文AbstractThegeneti

2、crelationshipandgeneticdiversityof20phylogeographyprovenancesofFraxinusManshuricaRupinNortheastofChinawerestudiedbyISSRmolecularmarkertechniqueByanalysingthegrowthcharatersof7yearoldFManshuricainthreetestsites，theprovena

3、nceselectionanddistrictwerebroughtforwardTheISSRmolecularmarkerresultswereasfollows1ISSRanalysisofgeneticrelationshipfor20provenancesofFManshuricacarriedoutwith11ISSRprimers108lociwhichcontaint104polymorphiclociwerefound

4、andthepercentageofpolymorphiclocisharedarangeof1852％～3796％ThatofProvenanceMSEWasthehighest(3796％)andProvenanceLSHwasthelowest(1852％)2GeneticvariationofFManshuricaWascountedinthispape(withShannoninformationindexandNei’Sge

5、nediversityindexTherangeofNei’Sindexwasfrom01644toO07590andthatofShannonindexwasfrom01103toO2375Thevariationsof20provenancesindicatedbythetwoindexeswereidentical3Thegeneticdiversity(Dst)，basedonNei’Smethod，amongprovenanc

6、esofFManshuricaWas0，1586Thecoefficientofgenedifferentiation(Gst)is06183TheestimateofgeneflowfromGst(Nmlis03086Thereare6183％and3817％oftotalvariationamongandwithinprovenancesrespectively4Geneticrelationshipclusterfigureof2

7、0provenancesof“FManshuricaWaSgoRenbasedonISSRmarkingtechniqueTheprovenancesofFManshuricahadbeendividedinto2populationsasfollowsThefirstgroupcontainedprovenanceYH，DL，SCZ，MES，HL，LJ，F(xiàn)Z，HUA，HR，andZH；andthesecondgroupcontaine

8、dprovenanceHUI，SL，DFH，TWH，WC，XFXL，WQ，WDZ，andLSHThere’SnosignificanceofcorrelativitybetweengeneticandgeographicaldistanceThroughinvestigatingthegrowthcharatersof7yearoldFManshuricageographicalvariances，theinteractionofgeo

9、graphicalvariancesandtestsites，andthegeneticstabilityofthe20provenanceswereanalyzedTheresultsofgrowthcharatersanalysisshowedthat：1AmongtheprovenancesofFManshurica，thereweresignificantdifferenceinthehighgrowthandtherewere

10、nosignificantdifferencesinrootdiametergrowth2TheheightgrowthandstabilitywerevariableamongprovenancesTheprovenanceSLwasthebestoneinthebothaspectsandwasselectedasthebestprovenance3TheinteractionofprovenanceandsiteWassignif