簡介：大連水產(chǎn)學(xué)院碩士學(xué)位論文北極礁膜的生物學(xué)及室內(nèi)培養(yǎng)的研究姓名李曉麗申請學(xué)位級別碩士專業(yè)水生生物學(xué)指導(dǎo)教師張澤宇20050610堡堡堅塑蘭塑蘭墨至宴堡莖塑竺壟STUDIESONTHEBIOLOGYANDINDOORCULTUREOFMONOSTROMAARCTICUMWITTROCKABSTRACTMONOSTRORAAARCTICUMISONEOFGREENALGAWITHIMPORTANTECONOMICVALUEINNORTHCHINATHEBIOLOGYANDINDOORCULTUREOFMONOSTROMAARCTICUMWASREPORTEDINTHEPAPERTHERESULTSSHOWTHATTHELENGTHOFTHETHALLIARRIVEDATTHEMAXIMUMPOINTOFL5CMAT3℃THERATEOFBODYCELLSTURNEDINTOGAMETANGIAARRIVEDATTHEMAXIMUMPOINTOF80％AT5。C；THELIBERATEDQUANTITYOFGAMETESARRIVEDATTHEMAXIMUMNUMBEROF267107／GAT58℃THENUMBEROFRELEASINGGAMETESREACHEDTOTHESUMMITINTHEEXTREMETIDEALARGENUMBEROFGAMETESWOULDRELEASEATTHELIGHTINTENSITYOF50001XANDATTHEWATERTEMPERATUREOFENHANCING2。CAFTERDRYINGGAMETOPHYTEINTHESHADEFOR14HOURSTHERATEOFCONJUGATIONARRIVEDATTHEMAXIMUMPOINTOF92％AT7。CTHEGERMINATINGRATEOFZYGOTEARRIVEDATTHEMAXIMUMPOINTOF85％AT10。CAND20001XAFTERDEALINGWITHZYGOTEINDARKFOR810HOURSTHEGERMINATINGRATEOFUNCONJUGATEDGAMETEWASBETWEENL％AND9％WHICHWASFARBEHINDOFTHATOFZYGOTEAT20“CAND3000IXZYGOTEGROWEDBEST111EDIAMETEROFZYGOTEWASINCREASINGFROM4PMTO4LPMWHENTHETHENATURALWATERTEMPERATUREWENTUPFROM5“CTO21℃ABOVE21“CZYGOTEGROWEDVERYSLOWLYANDTHEDIAMETEROFZYGOTEREMAINEDAT44PMAT23℃ZYGOTEBECAMELARGERWHENTHENATURALWATERTEMPERATUREWENTDOWNTO21℃ANDTHEDIAMETEROFZYGOTEREACHEDTO48／1卅WHENITDOWNT018℃THESUITABLELIGHTINTENSITYSHOULDBE600IXINTHEMATURATINGSTAGEOFZYGOTE，ALARGENUMBEROFSPORESWOULDRELEASEINTENSIVELYATTHELIGHTINTENSITYOF5000IXANDATTHEWATEL‘TEMPERATUREOFENHANCING2。CAFTERDEALINGWITHZYGOTEINDARKFOR12DAYSTHESUITABLENATURALWATERTEMPERATUREOFSPORESRELEASEINGRANGEDFROM17“CTO14℃ANDTHELIBERATEDQUANTITYOFSPORESARRIVEDATTHEMAXIMUMNUMBEROF61105／CM2AT15“CTHEGERMINATINGRATEOFSPORESARRIVEDATTHEMAXIMUMPOINTOF80％AT15。CAND3000IXAFTERDEALINGWITHSPORESINDARKFOR1014HOURSTEMPERATUREISTHEMAINFACTORWHICHAFFECTEDTHEGROWTHOFSPORELINGSPORELINGCOULDNOTSURVIVEAT206CAT20CAND30001XSPORELINGGROWEDBESTSPORELINGGROWEDFASTERWITHTHENATURALWATERTEMPETURELOWERSHELLWASMORESUITUABLEFORTHEGAMETESASTHESUBSTRATETHANTHATOFVINYLONROPEKEYWORDSMONOSTROMAARCTICUMGAMETE；ZYGOTE；ZOOSPORE；SPORELINGINDOORCULTUREIVSUPERVISORPROFZHANGZEYUMASTERCANDIDATELIXIAOLIMAJORINHYDROBIOLOGY

簡介：河北農(nóng)業(yè)大學(xué)碩士學(xué)位論文海水池塘養(yǎng)殖凈水芽孢桿菌的篩選、生物學(xué)特性及其應(yīng)用研究姓名高海英申請學(xué)位級別碩士專業(yè)發(fā)酵工程指導(dǎo)教師王占武20080601ISOLATIONANDSTUDYONBIOLOGICALCHARACTERISTICSOFWATERPURIFICATIONBACILLUSANDAPPLICATIONINMARINECULTUREPONDAUTHORGAOHAIYINGSUPERVISORWANGZATTANWUMAJORFERMENTATIONENGINEERINGABSTRACT11圮ACCUMULATIONOFORGANICPOLLUTANTS，AMMONIANITROGENANDNITRITENITROGENISTHEMAININDUCEMENTFACTOROFAQUACULTUREWATERDETERIORATIONDISEASESOCCURRENCEANDQUALITYDECREASING、析TLLFISHANDSHRIMPSTHEIMPLEMENTATIONOFBIOREMEDIATIONBYBENEFICIALMICROORGANISMHASBECOMETHERESEARCHANDDEVELOPMENTFOCUSATPRESENTTOSCREENANDGAINDOMINANTBACILLUSSTRAINSORTHEIRCOMBINATIONSWHICHHAVEEXCELLENTWATERPURIFICATIONEFFECTSANDPREPARATIONPERFORMANCEISTHECRUXTOFORMULATEEFFICIENTMICROECOLOGYWATERPURIFYINGPRODUCTS228BACILLUSSTRAINSWEREISOLATEDANDPURIFIEDFROMTHEMARINEPONDSEDIMENTTWODOMINANTSTRAINS，BACILLUST301ANDT905，WEREOBTAINEDTHROUGHTHESALTTOLERANCE，DEGRADATIONOFORGANICMATTERANDANAMONIAANDNITRITENITROGENSCREENINGTESTSNEFOURDAYSAMMONIADEGRADATIONRATEOFT301WAS5518％AND5200％ANDNITRITENITROGENDEGRADATIONRATEWAS5595％AND5152％SEPARATELYWHILE，AMMONIADEGRADATIONRATEOFT905WAS3525％AND3653％ANDTHENITRITENITROGENDEGRADATIONRATEWAS721O％AND7417％SEPARATELYWHENTHEINITIALAMMONIAANDNITRATENITROGENCONCENTRATIONWAS20MG／LAND40MG／LINSIMULATED療ESHANDSEAAQUACULTUREWATERBASEDONMORPHOLOGICALFEATURESANDRESULTSOFPHYSIOLOGICALANDBIOCHEMICALCHARACTERISTICS，T301ANDT905WASIDENTIFIED嬲BACILLUSSUBTILISACCORDINGTOTHEMANUALOFSYSTEMATICMETHODSOFDETERMINATIVEBACTERIALANDBERGEYSMANUALOFDETERMINATIVEBACTERIOLOGY硼KRESULTSOFSAFETYTESTSSHOWEDTHATTHETOXICITYOFTHETWOSTRAINSWASACTUALLYNONTOXICONMICEANDWHITE1EGSHRIMPS1F11ESTUDYOFTHEBIOLOGICALCHARACTERISTICSOFSTRAINSSHOWEDTHATTHET301ANDT905STRAINSCOULDUSEGLUCOSE。STARCHLACTOSE，MANNITOL，SUGARANDOTHERCARBONSOURCESN坨ORGANICNITROGENSOURCESOFPEPTONE，YEASTEXTRACTBEEFGAODENGWEREMORECONDUCIVEFORTHEGROWTHOFTHEBACTERIATHANINORGANICNITROGENSOURCE111ERESULTSOFTTESTSSHOWEDTHATK2HP04ANDKH2P04HADSIGNIFICANTEFFECTS011THEGROWTHOFBACILLUSSUBTILIST301ANDT905口005皿把SUITABLECONDITIONSFOR1R301ANDT905GROWTHWERECONSISTENTWITHWATERPURIFICATIONMAPPROPRIATETEMPERATURERANGEDFROM22“CTO38℃ANDPHVALUEFROM6T085111EINFLUENCEOFSALTCONCENTRATIONWASNOTSIGNIFICANTFORTHEIRGROWTHANDWATERPURIFICATIONTHEREFORE，T301ANDT905STRAINSCOULDBEUSEDFORBOTHFRESHANDSEA

簡介：華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文豬傳染性胃腸炎病毒S基因表達的研究及其原核表達產(chǎn)物的生物學(xué)活性分析姓名楊樂樂申請學(xué)位級別碩士專業(yè)預(yù)防獸醫(yī)學(xué)指導(dǎo)教師吳斌郭福生200505012002級碩士研究生學(xué)位論夕良好的反應(yīng)原性。以表達的融合蛋白為抗原初步建立了間接酶聯(lián)免疫吸附試驗EUSA方法；用方陣滴定確定其最佳抗原包被濃度分別是5／ZG／ML、1嘰G／ML，最佳血清稀釋倍數(shù)均為140。為了更好地消除非特異性反應(yīng)，增強表達產(chǎn)物的抗原性，利用桿狀病毒表達系統(tǒng)可進行翻譯后修飾等特點，我們試圖在SF9昆蟲細胞中進行目的基因的表達。目前已成功構(gòu)建了重組桿狀病毒穿梭表達載體BACMID．S1、BACMIDS1．2，為目的基因桿狀病毒表達的進一步研究奠定了基礎(chǔ)。本研究在TGEV病毒增殖的基礎(chǔ)上，克隆了TGEVS基因的兩個不同區(qū)段，構(gòu)建了原核表達載體并進行了表達，利用原核表達產(chǎn)物柙步建立了TGEV和PRCV的間接ELISA鑒別診斷方法。同時成功構(gòu)建了菱組桿狀病毒穿梭表達載體。這些工作為進一步研究TGEV和PRCV的流行和變異、開發(fā)新的診斷方法奠定了基礎(chǔ)，同時為其它冠狀病毒的研究提供了參考。關(guān)鍵詞TGEV{PRCV；S基因；原核表達；BAC．TO．BAC鑒別診斷

簡介：丁香因枝葉繁茂、花色淡雅而清香、耐寒、耐旱及土壤要求不高，因此是世界重要綠化、美化、香花樹種。丁香品種繁多，一些品種之間性狀比較相近，容易混淆。通過對包頭地區(qū)栽培的十四個品種進行生長發(fā)育習(xí)性指標(biāo)的調(diào)查和統(tǒng)計，研究結(jié)果表明1不同品種的葉芽發(fā)芽情況差異很大，花葉丁香和羽葉丁香發(fā)芽較晚，比暴馬丁香發(fā)芽期晚將近一個月。其他幾個品種發(fā)芽去相差不多，幾乎都在三月下旬到四月上旬。花葉丁香和羽葉丁香的發(fā)芽期與展葉期相接較近，約一周左右，其余的品種發(fā)芽期與展葉期間隔較遠，約十五天左右。2園內(nèi)丁香開花最早為紫丁香，約在5月1日左右，開花最晚的為暴馬丁香，約6月16日左右，其花期可延續(xù)在7月9日左右，園內(nèi)丁香的群體花期可達70天左右。3對開花期與花期接近的紫云丁香、羅蘭紫丁香、波峰丁香、什錦丁香、長筒白丁香、香雪丁香、紫丁香7個品種的花期做了單因素方差分析，品種間差異顯著，香雪丁香、波峰丁香與什錦丁香差異不顯著，其花期最長。羅蘭紫丁香與香雪丁香差異不顯著，羅蘭紫丁香、長筒白丁香與紫丁香差異不顯著。紫云丁香、長筒白丁香與紫丁香差異不顯著，其花期最短。4十四個品種中只有紫丁香、暴馬丁香、小葉丁香、遼東丁香和紅丁香正常結(jié)實，而其他種類不結(jié)實或偶爾結(jié)實。5丁香的落葉時間比較短，通常只有3到5天左右。丁香葉片全部能夠自然脫落，這也表明，它們能夠感知到光周期變化，對包頭地區(qū)的氣候狀況有較強的適應(yīng)能力。

簡介：7761【3博士學(xué)位論文論文趔II型醫(yī)型坐E她鋤歪叫曲墊鹽建絲口2壁一鹽I塑堂生始F￡盈塾筐一一F1‘女I韓寡|』1一J一一指1，救『『『J垃衛(wèi)當(dāng)盟控一學(xué)中IIIQH一～堋地J物翟一I巾牛L垸一二L趨11翌芒J逍一挺變I。IJ釘世五生互』JJ二』旦一浙江大學(xué)博士學(xué)位論文羅氏沼蝦兩種不同的熱休克蛋白70分子生物學(xué)特征和功能這種特殊的表達方式說明MARHSP70在羅氏沼蝦熱損傷保護中起著極其重要的作用，同時我們推測MARHSP70這種獨特的功能可能與其N一末端和C末端的缺失結(jié)構(gòu)相關(guān)。為了進一步分析這兩種分子在胞內(nèi)的表達情況，我們采用原位雜交和免疫組織化學(xué)在中樞神經(jīng)和肝胰腺兩種組織中進行了表達定位研究，結(jié)果表明MARHSC70和熱誘導(dǎo)后的MARHSP70在絕大多數(shù)細胞都有表達，尤其是在神經(jīng)分泌細胞和肝胰腺的R和F細胞中表達水平較高，并且熱誘導(dǎo)后的MARHSP70的表達水平遠高于MARHSC70，這些結(jié)果進一步表明MARHSP70在熱應(yīng)激保護中扮演著重要的角色，同時也說明熱應(yīng)激條件下，絕大部分細胞快速啟動這種基因的表達。為了進一步探討羅氏沼蝦這種誘導(dǎo)型MAR_HSP70的功能，我們構(gòu)建了MARHSP70一PET一28A原核表達系統(tǒng)，成功地表達了MARHSP70，發(fā)現(xiàn)在IPTG誘導(dǎo)5HR后，目的蛋白可達到細菌總蛋白的50％以上，同時通過免疫印跡技術(shù)證明了這種蛋白的免疫原性，而且通過優(yōu)化條件，采用低溫誘導(dǎo)，獲得了一定量可溶性MARHSP70。在MARHSP70功能分析中，我們建立了MARHSP70的活性檢測系統(tǒng)，在衛(wèi)COLIBL21DE3導(dǎo)入這一基因并誘導(dǎo)表達，發(fā)現(xiàn)能夠提高熱刺激后細菌的生存率，這說明M”一HSP70在一定程度上起到了對細菌的保護作用，這為誘導(dǎo)型HSP70S功能研究提供了很好的模型和思路?？傊@是第一次從蝦類中克隆到兩種熱休克蛋白70S基因，并研究了它們的表達特征及可能的功能，這兩個基因具有大多數(shù)物種HSP70S的共性，而更重要的是，它們自身的特殊性，這些特殊性對于認識水生無脊椎動物HSP70S的結(jié)構(gòu)及功能有著重要的理論價值和研究前景。關(guān)鍵詞羅氏沼蝦，熱休克蛋白70KDA，熱休克，表達分析和定位，原核表達，活性檢測

簡介：南京農(nóng)業(yè)大學(xué)碩士學(xué)位論文象耳豆根結(jié)線蟲生物學(xué)和分子生物學(xué)特征的研究姓名劉昊申請學(xué)位級別碩士專業(yè)植物病理學(xué)指導(dǎo)教師徐建華20050601關(guān)鍵詞象耳豆根結(jié)線蟲；寄主范圍；MTDNA；RDNAITS；RDNAIGS2；序列比較PCR檢測法

簡介：陜西師范大學(xué)碩士學(xué)位論文煙草超聲生物學(xué)效應(yīng)的研究姓名鄭媛申請學(xué)位級別碩士專業(yè)植物學(xué)指導(dǎo)教師肖婭萍20050501進細胞生長，而大劑量的處理則會造成不可恢復(fù)的影響超聲誘導(dǎo)3MIN的細胞，超聲后立即計數(shù)，其死亡率為67％，而超聲處理5MIN的細胞，處理后立即計數(shù)其死亡率卻為44％，處理7MIN的細胞。處理剛結(jié)束時的死亡率為72％；短時間的超聲處理3MIN可以促進愈傷組織細胞的生長，而長時間的處理誘導(dǎo)7RAIN，則使其生長明顯受到抑制。5MIN超聲處理愈傷組織的褐化率最低，而3RAIN和7MIN超聲處理后愈傷組織的褐化率都較高，尤其以7RAIN處理愈傷組織褐化最嚴重3種劑量的超聲都能使一定比例的細胞發(fā)生凋亡，效果相同。5適宜的低強度超聲波作用于植物細胞時，提高了細胞膜和細胞壁的穿透性，高強度超聲在液體媒質(zhì)中產(chǎn)生激烈而短暫的瞬態(tài)空化效應(yīng)。空化泡崩潰的瞬間，在空化泡周圍產(chǎn)生局部高溫高壓并伴有強烈的沖擊波，從而產(chǎn)生一系列物理化學(xué)效應(yīng)1從細胞的表面結(jié)構(gòu)來看，則7RAIN處理對細胞結(jié)構(gòu)損傷最大，產(chǎn)生許多空化泡狀結(jié)構(gòu)。細胞團周邊細胞的表面變得粗糙，出現(xiàn)許多不規(guī)則顆粒突起，細胞核質(zhì)相混、胞質(zhì)破裂，甚至固縮，并出現(xiàn)大吞噬泡，細胞壁嚴重扭曲，質(zhì)壁分離更加嚴重，有的細胞幾乎不含內(nèi)含物。2經(jīng)超聲處理3MIN后的細胞結(jié)構(gòu)模糊，液泡化程度增加，有的細胞核和細胞質(zhì)被大液泡擠到細胞邊緣，細胞器更少。細胞壁扭曲、變形，發(fā)生部分質(zhì)壁分離。煙草懸浮細胞經(jīng)超聲處理7MIN后，電鏡觀察表明，細胞質(zhì)內(nèi)出現(xiàn)新的小體，細胞內(nèi)含物減少，液泡化程度增加；細胞壁嚴重扭曲，質(zhì)壁分離更加嚴重，細胞核產(chǎn)生不規(guī)則微核。關(guān)鍵詞煙草；組織培養(yǎng)；超微結(jié)構(gòu)；超聲N

簡介：湖南農(nóng)業(yè)大學(xué)碩士學(xué)位論文螟黃足盤絨繭蜂支序系統(tǒng)學(xué)研究及菜粉蝶盤絨繭蜂生物學(xué)特性研究姓名王常平申請學(xué)位級別碩士專業(yè)農(nóng)業(yè)昆蟲與害蟲防治指導(dǎo)教師游蘭韶20050601ABSTRACTBASEDONMORPHOLOGICALSTUDIES70CHARACTERTRANSFORMATIONSERIESOF5SPECIESOFCOTESIAFLAVIPESCOMPLEXASINGROUPAND1SPECIESOFMICROPLITISANDDILOLCOGASTERASOUTGROUPRESPECTIVELY，67CHARACTERTRANSFORMATIONSERIESOF5SPECIESOFCOTESIAFLAVIPESCOMPLEXASINGROUPAND3SPECIESOFTHESAMEGENERAOUTGROUPWERESELECTEDRESPECTIVELYFORCLADISTICANALYSISWITHPHYILPSOFTWAREBASEDONTHEMETHODOFCLADOGRAMSINANATTEMPTTOASSESSTHEPHYLOGENETICRELATIONSHIPSAMONGTHECOTESIAFAVIPESCOMPLEXTHERESULTSOFPHYLOGENETICCLADOGRAMINDICATEDTHATCFLAVIPESCOMPLEXISAMOMOPHYLETICGROUPANDTHATCCHILONISANDCSESAMIAEAREMORECLOSELYRELATEDCCHILOLUTEELLIANDCHANSHOUENSISAREMORECLOSELYRELATEDTOOCFLAVIPESISASPECIESTOBEINDEPENDEDUPONABOVE4SPECIESINCONCLUTIONWEDOWHATEVERTHERELATIVEGENUSASOUTGROUPORTHERELATIVESPECIESASOUTGROUPTHERESULTSCANCORRECTLYREFLECTTHERELATIONSHIPSAMONGTHESPECIESOFCOTESIAFLAVIPESCOMPLEXONTHEOTHERHANDTHROUGHFEEDINGTHELARVAOFPIERISRAPAEANDITSPARASITICNATUREENEMIESCOTESIAGLOMERATALINDOORWESTUDIEDTHEBIONOMICOFCOTESIAGLOMERATARESULTSSHOWTHATCOTESIAGLOMERATAOCCURS4GENERATIONSAYEARINTHEAREAOFCHANGSHATHETIMEISFROMLATEAPRILTOMIDJULYFOURPEAKSOFTHELARVAOFPIERISRAPAEBYCOTESIAGLOMERATAWERESERVEDEARLYMAYEARLYJUNELATEJUNEANDEARLYJULYRESPECTIVELYTHEPARASITIZATIONRATERANGEDFROM184889THETOTALDEVELOPMENTTIMEFROMEGGTOADULTRANGEDFROM135DAYSAT2940CTO162DAYSAT2030CCOTESIAGLOMERATASELDOMPARASITZED4TH5THINSTARLARVAEANDNOTPARASITZEDITHBUTPREFERREDSECONDINSTARLARVEHONEYSOLUTIONCANLENGTHENTHEADULTLONGEVITHAPPARENTLYKEYWORDCOTESIARAPAEBIONOMICCOMPLEXPHYOLGENYCLADISTICSCOTESIAGLOMERATAPIERIS

簡介：南昌大學(xué)碩士學(xué)位論文背角無齒蚌寄生蚌螨種群生物學(xué)及內(nèi)部結(jié)構(gòu)研究姓名焦玉壇申請學(xué)位級別碩士專業(yè)水生生物學(xué)指導(dǎo)教師文春根20071224ABSTRACTABSTRACTPOPULATIONDYNAMICSOFTHEWATERMITEUYPSILOPHORAANDUARCUATAWASINVESTIGATEDINFRESHWATERBIVALVEA．WOODIANAWOODIANADURINGTHEPERIODFROMAUGUST2006TOJULY2007INNANCHANG．APARERNOFSEASONALVARIATIONWASOBSERVED，WITHPREVALENCEANDABUNDANCEOFUYPSILOPHORAANDUARCUATAPEAKEDINMAYANDJUN，RESPECTIVELY．THENUMBEROFMITESININDIVIDUALHOSTWASSIGNIFICANTLYCORRELATED謝THTHESIZE，BUTNOTWITHTHESEX，OFBIVALVES．THEUYPSILOPHORAANDUARCUATAHAVEASITEPREFERENCEFORMANTLESANDGILLOFTHEHOST，RESPECTIVELY．ANALYSISFORINTERSPECIFIEASSOCIATIONINDICATEDTHATITWASOBVIOUSLYNEGATIVEASSOCIATIONBETWEENUYPSILOPHORAANDUARCUATA；ITWASPOSSIBLETHATTHEYWEREPARASITICONDIFFERENTPOSITIONSORTHEECOLOGICALNICHESISOLATED．NLEPHOTOTAETICBEHAVIOROFUYPSILOPHORA,UARCUATAANDUPENICILLATUSWERESTUDIEDBYARTIFICIALEQUIPMENT．THEPHOTOTACTICINDEX，WHICHHADBEENDETECTEDUNDERTHECONDITIONSOFDIFFERENTLIGHTINTENSITYANDVARIEDCOLOURLIGHT，WASCONSIDEREDASALLIMPORTANTPERFORMANCEINPHOTOTACTICBEHAVIORRESEARCH．THERESULTINDICATEDTHATTHEREWASSIGNIFICANTDIFFERENCEINALLOFTHREESPECIESMITESPN∽．INALLLIGHTINTENSITYEXCLUDED700LUX，THEREWEREEXTREMELYSIGNIFICANTDIFFERENCEPNDNFORUYPSILOPHORA,WITHTHEINCREASEOFLIGHTINTENSITY,THEPHOTOTACTICINDEXINCREASED；UPENICILLATUSTHEPHOTOTAETICINDEXREACHEDTHEMAXVALUEONTHE1000LUX；WHILEUARCUATAPERFORMANCEDDIFFERENTLYWITHTHEFORMERTWOSPECIES，WHICHSHOWEDPOSITIVEPHOTOTACTICON700LUXWHILEPERFORMANCEDNEGATIVEPHOTOTAETICUNDEROTHERELSEONES．HOWEVER,UARCUATASHOWEDPOSITIVEPHOTOTAXISINRED．BLUEANDYELLOWLIGHT,WHICHBECOMEMORESENSITIVEUNDERREDLIGHTTHANBLUEANDYELLOW．UPENICILLATUSHOLDEDPOSITIVEPHOTOTAXISONLY,WHILEUNDEROTHERCOLOURLIGHTBECOMESNEGATIVEPHOTOTAXIS．UYPSILOPHORAPERFORMANCEDPOSITIVEPHOTOTAXISUNDERREDLIGHTONLY．THEDIGESTIVESYSTEMANDGONADOFADULTUARCUATAAREDESCRIBEDFROMSERIALPARAFFINSECTIONS．THEALIMENTARYCANALCONSISTSOFPHARYNX，STOMACH,MESENTERON,III

簡介：獨創(chuàng)性聲明本人聲明所呈交的論文是我個人在導(dǎo)師指導(dǎo)下進行的研究工作及取得的研究成果。盡我所知，除了文中特別加以標(biāo)注和致謝的地方外，論文中不包含其他人已經(jīng)發(fā)表或撰寫過的研究成果，也不包含為獲得寧夏大學(xué)或其它教育機構(gòu)的學(xué)位或證書而使用過的材料。與我一同工作的同志對本研究所做的任何貢獻均已在論文中作了明確的說明并表示了謝意。研究生櫞至乳時間五，卜／年Y只兩日／關(guān)于論文使用授權(quán)的說明本人完全了解寧夏大學(xué)有關(guān)保留、使用學(xué)位論文的規(guī)定，即學(xué)校有權(quán)保留送交論文的復(fù)印件和磁盤，允許論文被查閱和借閱，可以采用影印、縮印或掃描等復(fù)制手段保存、匯編學(xué)位論文。同意寧夏大學(xué)可以用不同方式在不同媒體上發(fā)表、傳播學(xué)位論文的全部或部分內(nèi)容。保密的學(xué)位論文在解密后應(yīng)遵守此協(xié)議研究生簽名凄嘭眇導(dǎo)師簽名許夠時間力，．√帆二∥年歲乒’日F，一Q年J刪日／F摘要針對寧夏中部干旱區(qū)砂田西瓜廣泛種植，土地利用結(jié)構(gòu)改變導(dǎo)致的西瓜連作障礙廣泛存在的現(xiàn)象，設(shè)計了西瓜一甜瓜_西瓜M、西瓜_大豆÷西瓜S、西瓜_辣椒_西瓜P、西瓜_油葵_西瓜O4種輪作模式，研究四種輪作下土壤化學(xué)性狀、土壤微生物學(xué)性狀及土壤微生物功能多樣性等指標(biāo)的變化規(guī)律，探討了輪作模式調(diào)控連作障礙的可能作用機制。主要研究結(jié)果如下1、持續(xù)砂田連作使得引起西瓜枯萎病發(fā)生的鐮刀菌數(shù)量和枯萎病病情指數(shù)呈上升趨勢，土壤PH也隨著連作年限的增逐年升高，而土壤肥力總體呈現(xiàn)下降趨勢，西瓜產(chǎn)量在連作栽培條件下持續(xù)下降。2、持續(xù)砂田連作條件下西瓜根際土壤微生物總生物量、細菌數(shù)量、放線菌數(shù)量和細菌／真菌的值呈下降趨勢，而真菌數(shù)量逐年上升；土壤微生物生物量碳、氮和土壤微生物呼吸強度隨著連作年限的增加逐年下降持續(xù)連作改變了土壤酶的活性，隨著連作年限的增加土壤脲酶和蔗糖酶逐年下降，土壤過氧化氫酶先上升后下降，土壤脲酶先下降后上升。3、輪作模式下，西瓜根際土壤PH均低于連作，土壤各養(yǎng)分較連作有不同程度的提高，其中M、S、P土壤有機質(zhì)、全氮、堿解氮、全磷、速效磷在輪作年際間均顯著增加。4、輪作模式下，西瓜根際土壤微生物總生物量、細菌數(shù)量和放線菌數(shù)量均高與連作，真菌數(shù)量顯著低于連作，輪作兩年間B／F的大小依次為PMSOCK；土壤酶活性各輪作模式較連作均有所提高，其中P增加顯著；M、S、P顯著提高了西瓜根際微生物碳、氮、微生物熵和土壤基礎(chǔ)呼吸強度。輪作模式P、S、M改變了西瓜根際微生物群落對主要碳源的利用強度，以P的增幅更為顯著，培養(yǎng)168H時，P、S、M的AWCD值分別比連作升高了70．25％、2．19％、10．67％，輪作模式O卻降低了西瓜根際土壤微生物碳源轉(zhuǎn)化效率。砂田輪作后，經(jīng)過田間調(diào)查測產(chǎn)發(fā)現(xiàn)，西瓜枯萎病發(fā)生的鐮刀菌數(shù)量和枯萎病病情指數(shù)較連作有所下降，西瓜產(chǎn)量量較連作增加。P、S、M輪作模式被認為是有利于改善砂田西瓜連作栽培根際微生物環(huán)境，緩解西瓜連作障礙的栽培模式關(guān)鍵詞西瓜，連作，輪作，病害，平均顏色變化率AWCD

簡介：南京林業(yè)大學(xué)碩士學(xué)位論文楊樹爛根病病原生物學(xué)特性及與環(huán)境因子的關(guān)系姓名陳先中申請學(xué)位級別碩士專業(yè)生態(tài)學(xué)指導(dǎo)教師張銀龍20050601STUDYONPATHOGENANDBIOLOGICCHARACTERISTICSOFTHEROTROOTSDISEASEOFPOPLARANDTHERELATIONBETWEENTHEDISEASEANDFACTORSOFENVIRONMENTSABSTRACTTHEARTICLESUMMARIZETHELATESTSTBDYONTHEDISEASEOFROOTOFPOPLARTHECLASSICSYSTEMSOFFUSARIUMANDTHERELAFIONBETWEENTHEDISEASEANDFACTORSOFENVIRONMENT，ANDSTUDYCONCRETELYANEWDISEASEOFPOPLARROTROOTSDISEASEOFPOPLARWHICHOCCURSONTHENORTHOFJIANGSUPROVINCERECENTLYRTHERESULTSAREASFOLLOWS1THEA】鼢OFTHEDISEASEANDTHEIMPORTANTSYMPTOMSOFTHEDISEASEREPORTEDLYTHEROTROOTSDISEASEOFPOPLAROCCURSONALARGESCALEAREAOFTHENORTHOFJIANGSUPROVINCEMOSTLYTHEIMPORTANTSYMPTOMSOFINFECTEDSEEDLINGOFPOPLARASFOLLOWS④NEDISEASEHARMSTHEITO3YEARSOLDSEEDLINGSMOSFLY，THEINFECTEDSEEDLINGS’ROOTGROWBLACKANDROT，MANYBLACKSPOTSONTHETWIGS，SOMEBLACKSPOTSLINKSTRIP，ANDTHEBUDSDEADFORLOSTWATER窯THECUTTINGSROTANDSEEDLINGSPRODUCTIVITYDESCENDS2THEISOLATION，INOCULATIONANDFILTRATIONOFPATHOGENICANDPATHOGENICITYTESTTHROUGHISOLATINGFUNGUS，INOCULATIONANDPATHOGENICITYTEST，THEDCF4ANDDCF8FUNGUSPATHOGENICITYARESTRONGERTHANTHEOTHERS，THEYAREIDENTIFIEDTOTHEPATHOGENICFUNGUSBELONGSTOWHICHKINDFUNGUSANDNALTLETHENEWDISEASE3IDENTIFICATIONOFPATHOGENICFUNGUSOFROTROOTSDISEASEOFPOPLAR’TRTROU曲OBSERVATINGANDMENSURATINGTHEGROWTHSPEEDOFCOLONYTHECOLORTEXTUREANDCOREOFTHECOLONYTHECHARACTERISTICSOFCONFIGURATION。THESEXUALPHASE，THEPRODUCINGCELL，THEPATHOGENICFUNGUSISIDENTIFIEDTOBELONGTODISCOLORGROUPANDFUSARIUMHETEROSPORUMWITHTHEBOOTHSYSTEM4THEINFLUENCEOFENVIRONMENTFACTORSTOPATHOGENOFFUSARIUM①THEFUNGUSGROWSRAPIDLYCULTIVATINGFROM24TO26“CANDGROWTHSTOPAT4AND400THELARGECONIDIACANGERMINATEFROM15TO35℃MODERATEANDCANNOTGERMINATEABOVE40℃ANDBELOW4℃②THEFUNGUSCANNOTGROWATPH22ANDS乜ONGALKALESCENCE，ANDGROWOPTIMUMATPH80THESPORECANGERMINATEPHFROM50TO100PH8090OPTIMUM⑧LJGHTHAVEUOMUCHEFFECTONTHEGROWTHOFFUNGUSMYCELIUMANDSPOREGERMINATION緲THEPATHOGENICITYISSIMILARATKINDSHUMIDITYABOVE90％，ABOUT80％⑨FUNGUSMYCELINMANDSPOREENDURELOWTEMPERATUREWELL，BUTTHEENDURANCETOHI曲TEMPERATUREARENOTENOUGH5MENSURATINGANDANALYZINGTHEPHYSICSANDCHEMICINDEXOFTHESOIL，ANDACQUIRINGTHEMICROBETOTALITYANDFUSARIUMQUANTITYINTHESOILⅡ

簡介：中山大學(xué)博士學(xué)位論文R768138水稻第6染色體S5區(qū)基因克隆及分子生物學(xué)研究專業(yè)遺傳學(xué)博士生仲健導(dǎo)師王金發(fā)教授主席力膨≥委員釤7彩犯≠掀式釤澎吾五1皴力廣州中山大掌生命科掌掌院二00五年五月十八日仲健中山大學(xué)博士學(xué)位論文水稻第6染色體SS區(qū)基因克隆及分子生物學(xué)研究過RTPCR檢測發(fā)現(xiàn)這些轉(zhuǎn)基因株的OS6AP1基因的轉(zhuǎn)錄本在體內(nèi)的水平大大降低。RTPCR分析表明以硎尸』基因在野生型植株的多個組織中表達，但在花粉中未能檢測到其表達。這些結(jié)果表明OS6AP1基因的功能是調(diào)節(jié)水稻根系的發(fā)育，并影響株高和開花期。OSDUFL296基因由10個內(nèi)含子與11個外顯子組成，推算的編碼蛋白由854個氨基酸殘基組成，并預(yù)測在蛋白質(zhì)N一端含有一長約60個氨基酸的名為DUFL296的保守結(jié)構(gòu)域，RTPCR分析表明該基因在所有檢測的組織中都有表達，包括花、葉、莖、根、幼穗等，而且表達量較為均勻。通過RNA沉默，發(fā)現(xiàn)經(jīng)RNA沉默的轉(zhuǎn)基因水稻表現(xiàn)出較慢的生長速率，目前轉(zhuǎn)化小苗表型變化仍在進一步觀察中。OSDSP基因序列分析表明OSDSPL基因克隆全長1119BP，推測的OSDSPL基因產(chǎn)物由225個氨基酸組成。EDNA序列與基因組序列分析表明該基因由5個外顯子及4個內(nèi)含子構(gòu)成，多個在線分析工具表明該蛋白質(zhì)屬于雙特異蛋白磷酸酶家族。我們將該基因序列提交NCBI數(shù)據(jù)庫，得到接受并獲得序列號AY669072。關(guān)鍵詞水稻ORYZASATIVAL；廣親和品種WCVRNA沉默；OSGAP1GPI一錨定蛋白；LYSM結(jié)構(gòu)域；OSDSP；OSDUFL296

簡介：吉林農(nóng)業(yè)大學(xué)碩士學(xué)位論文輻射敏化劑對激光照射向日葵生物學(xué)效應(yīng)的影響姓名王新風(fēng)申請學(xué)位級別碩士專業(yè)作物遺傳育種指導(dǎo)教師樸鐵夫20050601吉林農(nóng)業(yè)大學(xué)碩士學(xué)位論文輻射敏化荊對激光照射向日葵生物學(xué)效應(yīng)的影響增加，畸變率也在增加；有絲分裂指數(shù)下降，抑制細胞分裂，胚根長度降低。所有這些效應(yīng)在激光處理20MIN時，誘變效應(yīng)最大。2、用輻射敏化劑EDTAIMMOL／L及丙烯酰胺5MFITOL／L單獨處理向日葵種子，處理時間分別為3H、5H和7H。結(jié)果發(fā)現(xiàn)三種處理均誘發(fā)了染色體畸變，且畸變率隨處理時間的增加而加大，染色體畸變類型以橋為主；有絲分裂指數(shù)和胚根長度降低。在處理7H時畸變效應(yīng)最大。由此，在使用輻射敏化劑時應(yīng)考慮到它的誘變效應(yīng)。3、先用NDYV04激光前處理，時間為5MIN、10MIN、15MIN，之后用EDTA和丙烯酰胺后處理，時間為3H、5H和7H。結(jié)果表明復(fù)合處理組合的染色體畸變率均明顯高于相對應(yīng)的激光單獨處理組合，有絲分裂指數(shù)和胚根長度隨處理時間的增加而降低。4、對SDYV04激光處理向日葵的萌發(fā)種子的染色體畸變情況進行了統(tǒng)計，同時與處理向EL葵干種子的染色體畸變情況進行了比較。結(jié)果發(fā)現(xiàn)NDYV04激光處理向日葵種子的染色體畸變情況是處理千種子的大于處理萌發(fā)種子。關(guān)鍵詞輻射敏化劑NDYV04激光EDTA丙烯酰胺向日葵輻射誘變

簡介：分類號S186密級Y8專279S單位代碼10749學(xué)號0202117寧夏大學(xué)學(xué)位論文甘草螢葉甲生物學(xué)、生態(tài)學(xué)特性及綜合防治的研究STUDIESONBIOLOGICALANDECOLOGICALCHARACTERISTICSANDINTEGRATEDCONTROLOFDIORHBDATARSALISWEISE研究生韭漁攔指導(dǎo)教師揚墅霞班究雖申請學(xué)位門類級別農(nóng)學(xué)專業(yè)名稱壅些昆蟲皇塞座隨擅研究方向基蟲生盔皇綾金隨漁所在學(xué)院壅堂瞳2005年5月ABSTRACTDIORHBDNTARSALISWEISEISONEOFTL∞MOSTPESTSTOLEAVEOFGLYCYRRHIZAUR出ENSISFISCKITHADCAUSEDHUGELOSSIN2003AND2004,THEPSPERBADSTUDIEDSYSTEMATIDYTHEMOFPHIED刪Ⅻ柵蛐五對IFEHISTOFYHRESBULDTEMPERATURE,EFFECTIVEA∞ⅢM蜥VE鈀M非棚缸I地ANDSPECIALDISTNBUTIONP矗T把MBYROOTLMADFIELDEXPERIMENTSHIRANGEOFL5～3CC，ITHADBEENINVESTIGATEDTHEGROWTH,SOFVIVAL,WOPAGATIONANDSOFORTH．MEANTIME．THELIFETABLEOFEXPE越ENTALPOPLL蜥GNWASESTABLISHEDMLATXRAURYATDIFFERENTTEMPERATURESANDTHEEFFECTIVESYNTHETICCONTROLMETSL．1REWSSDRAWNUPPRELIMINARILY．THERESUITSALE8SFCLLLOWS】BIOLOGICALCH鋤D【ARSTLLIS證SECTOOGUR2OR3GENERATIONSINOⅢ1EYE盯．TBEADULTOFTHELASTG豇LE蒯帆START幻LIVEⅡLRDI培HWINTERBELOWCENTIMETRE2～3INEANTL．NEXTYEA‘訥THESECEODORLASTTENDAYSOFAPRIL，THEADULTAPPEARAGAINANDBESINTOEAT,MATEM】DLAYEGGS．MOSTEGSSW啪LAIDINTHEBACKOFLEAVEORINTHESOIL．NMLARVEHASTHREEDEVELOPMENTALSTA辨S．ADULTANDLARVAALLEATLEAVE．THEYHOLYEATLEAVEOFG／YCY衲IZAURALEMISFISCH．ADULTHASTHED目MC嘧OFFEIGNDEATH．ITRAFTL毋INSHCFTDISTANCE．FURTHERMORE,ADULTCMMATEMADRAYMANYTIMESALLTHELIFE．2EFFECTIVEACCUMULATIVETEMPERATMEINTHERANGEOFL5～36“C．THEGROWTHSPEEDOFDIOR／坊DATARSALISWEISEGOESUPWITHTHERISINGOF哪ATURE．NMRELATIONSHIPOFGROWTHSPEEDANDTEMPERATOFEACCORDSTOLOGISTICMODEL．ANDWEGOTTHETHRESHOLDTEQP∞NJMANDTHEEFFECTIVEACCUMULATIVETEMPERATOFEOFEACHDEVELOPMENTALS0眥SRESPEETIWAY．3SPATIALDISLN‘BULIONBYESTIMATEDWITHTHESUITABLEDEGREEOFSPA砌DISTRIBUTIONANDX2TE雞SPA6．ALDISTRIBUTIONPA帥OFADULTCCNFCNNED幻EILHERTHEPATTERNOFAGGREGATION啊THENEGATIVEBMOMIALDISTRIBUTIEN,SPATIALDISTRIBUTIONPAMMAOFLARVEONLYCONFORMEDTOTHENEGATIVEBINOMIALDI姐BUTIONITWASALSOTESTEDTHATTHEDISTNLOUTIONPATTERNOFLARVEANDADULTW啪ALLAG刪VEBYASSREGATIAAINDEXESINCLUDINGIVALUE,M‘／MVALUE,CAVALUE，DIFFUSIVEEOE伍EIEAT,KVALUE．ITWASFOFTLLEAEONFOFMEDBYM。111REGRESSIONANALYSISLAWANDTOYL口POWEFRULE．MEANTIME,TBEMODELSOFTBEOFETICAL∞MPLILLGOFADULTANDARVEHADBEENSETUP,TOO4EFFECTOFTEMPEMTURETOSURVIVETBESINVIRALRATEOFEACHDEVDOPMENTALSTAGEFORM23“12T030“2ISHIGHERANDTBEHIGHESTISAT25“12．WHENTEMPERATUREISLOWERTHAN23℃ORISHIOMRTHAN30“C,STAVIVALRATEDROPGRADUALLY．INPREOVIPOSITION,THEORDEROFACCLWNULATIVESURVIVALRATEFROMHIGHTOLOWIS25℃，27℃，23℃，3032，21℃，33℃，18℃，36“2，15“C。5EFFECTOFTEMPERATURETOEGGIM3DNEFIENSINTHERANGEOFTEMPERATMFROM18CTO36“C，EGGPRODNCTIENSISTHEHI,BESTAT25℃MAD27“2．WHENTHETEMPERAANEISHIETHAN27“COFISLOWERTHAN25“2，EGGPRODUETIMZSDECREASEGRADUANY．MMEOVER,WITHTHERIS噸OFTEMPERATURE,THEDATEOFLAYINGEGGSISMOREEARLY,THEPERIODOFL咖EG擎ISMORESHORT,BUTTHERA，19EOFCHANGESISHTTLEGRADUALLY．AT15℃THISINSECTDONOTLAYEGGSANYLONGER．6EFFECTOFTEMPERATURETOADULTLIFESPANHITHE目RLGEOFTEMPERATOFEFROM18“12TO362，WITHTHERISINGOFTEMPERATOFE,THEADULTLIFESPANBECOMESMOFEANDMORESHORT．ATTHES∞∞TIME,THELOGISTICMODEL吣SETUPABOUTTHERELATIONSHIPB盹WEENADULTLIFESPAN柵DTEMPERMUREN0．1093／1EXPⅢ

簡介：委糾犬學(xué)碩士學(xué)位論文2005屆家蠶成品卵微粒子病分子生物學(xué)檢測技術(shù)的研究STUDYONTHEMETHODOFMOLECULARBIOLOGICALDETECTIONOFPEBLINCOFBOMBYXMORIEGGS研究生姓名指導(dǎo)教ⅡⅡ姓名專業(yè)名稱研究方向論史提交H期潘中華責(zé)成良教授特種經(jīng)濟動物飼養(yǎng)家蠶病理學(xué)廈病毒分子生物學(xué)2005年5月蘭塑堂竺坐竺些型墮嬰型竺皇塑型塑塑墮￡竺垡皇竺皇堅竺竺璺璺苧一竺坐型一STUDYONTHEMETHODOFMOLECULARBIOLOGICALDETECTIONOFPEBRINEOFBOMBYXMORIEGGSABSTRACTTHESTUDYFOCUSEDONSEQUENCEFEATURE，MOLECULAREVOLUTIONOF16SRRNAGENEOFNOSEMABOMBYCTS，ANDTHEREFORETHEPCRMETHODTODETECTPEBRINEOFBOMBYXMORLEGGSWASCREATEDTHE16SRRNAGENEWASCLONEDTHROUGHPERWITHNOSEMABOMBYCTSDNATEMPLATEANDASPECIALPAIROFPRIMERSFOR16SRPDQAGENEOFNOSEMABOMBYCTSSEQUENCEANALYSFSSHOWSTHATTHENUCLEOTLDECONSISTSOF1235BPLACKINGOFSEVERALREGIONSWHICHA∞CONSIDERED∞EUKARYOTICSEQUENCES。ANDITSSEQUENCESTRUCTUREFEATUREISMORESIMILARTOTHATOFPROKARYOTICITWASPRESUMEDTHATINVERYEARLYSTAGENOSEMABOMBYCIADIVERGEDFROMPROKARYOTESMOLECULAREVOLUTIONANALYSISSHOWSTHATMLCROSPORESBELONGSTOTHESAMEGENUSMAYLOCATEDIFFERENTBRANCHESONPHYLOGENTICTREETHERESULTSOFDNAEXTRACTIONSFROMPUREMLCROSPORESOFNOSEMABOMBYETSDEMONSTRATETHAT13XLO～PGDNACALLHEEXTRACTEDFROMONEMTCROSPORE。AND325XI釅PGDNA，SCILICET4MLCROSPURES，M25PLPCRSYSTEMISTHETHRESHOLDFORSUCCESSFULDETECTIONOFPCRTHERESULTSOFDNAEXTRACTIONFROMSILKWORMEGGSWITHPEBNNESHOWTHATTOTALDNANORMALLYEXTRACTEDFROMSILKWORMEGGSWLTHPEBNNECANHEUSEDTODETECTPEBNNEAMONGSDKWORMEGGSWHENINCUBATIONEGGSWEREPREPAREDWITH30％KOHPRACTICALEXAMINATIONRESEARCHILLUSTRATESTHATPCRCARLSENSITIVELYDETECTIⅪBRMEFROMONESILKWORMEGGANDTHEMOSTSENSITIVEFORPEBNNEDETECTMNISONEPEBRMEEGGMIXEDWITH300NORMALEGGSTHEPROBABILITYOFIDENTIFYINGONEPEBRMEEGGAMONG100NORMALEGGSISABOUT80％THEMANIPULATEREGULATIONANDEVALUATIONSTANDARDOFPCREXAMINATIONFORTHEPEBRLNERATIOAMONGSILKWORMEGGSWERECREATEDBASEDONALLOFTHEEXPERIMENTALDATAKEYWORDSSILKWORMBOMBYXMOLLNOSEMABOMBYCLS，THEPRODUCTEGG，RATEOFDISEASEEGG，16SRRNA，PCRDETECTIONUWRITTENBYPANZHONGHUASUPERVISEDBYGONGCHENGHANGAJ～，，、J一；；～。，孓，，L