不同時(shí)間游泳運(yùn)動(dòng)對(duì)大鼠睪酮合成的影響及機(jī)制研究.pdf

1、楊洲洲不同時(shí)間游泳運(yùn)動(dòng)對(duì)大鼠睪酮合成的影響及機(jī)制研究中文摘要實(shí)驗(yàn)?zāi)康模罕狙芯客ㄟ^(guò)對(duì)雄性大鼠進(jìn)行不同時(shí)間的有氧運(yùn)動(dòng)實(shí)驗(yàn)干預(yù)，觀(guān)察分析機(jī)體內(nèi)重要睪酮合成酶和雄性激素變化情況，進(jìn)而探究不同時(shí)間有氧運(yùn)動(dòng)對(duì)睪酮合成機(jī)制的影響。為嘗試解釋和減少運(yùn)動(dòng)員運(yùn)動(dòng)性低血睪酮現(xiàn)象，消除運(yùn)動(dòng)疲勞，提高運(yùn)動(dòng)員運(yùn)動(dòng)能力提供一定理論支持。實(shí)驗(yàn)方法：將37只健康的雄性SpragueDawley(SD)大鼠隨機(jī)分為3組，實(shí)驗(yàn)結(jié)束后各組還剩：正常對(duì)照組(C，n=8)、普通運(yùn)

2、動(dòng)組(CE，n=lO)、長(zhǎng)時(shí)間運(yùn)動(dòng)組(LE，n=lO)，各組統(tǒng)一普通飼料喂養(yǎng)。運(yùn)動(dòng)方案為適應(yīng)性游泳1周，C組無(wú)訓(xùn)練，CE組60min，LE組120min無(wú)負(fù)重游泳運(yùn)動(dòng)，每周6天，休息一天。實(shí)驗(yàn)結(jié)束后采用ELISA法測(cè)定組織睪酮(T)、313一HSD、P450c17、17B—HSD；ELISA法測(cè)定血清中睪酮(T)、瘦素(LEP)、FSH、LH含量；用RTPCR檢測(cè)大鼠睪丸組織中313_HSD、P450c17和17B’HSD的表達(dá)量：胍染

3、色觀(guān)察睪丸組織形態(tài)學(xué)的變化。實(shí)驗(yàn)結(jié)果：1睪酮合成關(guān)鍵酶313HSD在睪丸組織中的含量與CE組、LE組與C組相比均呈現(xiàn)顯著降低(P005)，LE組則差異極為顯著(P005)，LE組則有顯著性差異(PO05)。3血清中瘦素(LEP)、LH、FSH與對(duì)照組相比均有顯著性變化，瘦素(LEP)呈現(xiàn)顯著升高趨勢(shì)(P005)，而LH、FSH則有顯著下降現(xiàn)象出現(xiàn)(P005)。運(yùn)動(dòng)前各組大鼠體重?zé)o顯著性差異，經(jīng)10周運(yùn)動(dòng)后兩，運(yùn)動(dòng)組大鼠體重與對(duì)照組相比均

4、有極顯著性差異(P001)。10周后與C組相比CE組睪丸系數(shù)有極顯著性差異(P001)，而LE組有顯著性差異(P005)。楊洲洲不同時(shí)間游泳運(yùn)動(dòng)對(duì)大鼠睪酮合成的影響及機(jī)制研究Thepurpose：AbstractlILlIlllUrollIIllllIIIIIIIIIIImlIllIllIY3130764Thisstudybasedonthemaleratexperimentindifferenttimeofaerobicexercis

5、eintervention，observationanalysisbytheimportantandchangeofthemalehormonetestosteronesynthetase，andtoexplorethedifferenttimeaerobicexerciseinfluenceontestosteronesynthesismechanismTotrytoexplainandreducetheathletes’exerci

6、se—inducedlowerbloodtestosteronephenomenon，eliminatefatigue，improveathletesportsabilitytoprovidecertaintheoreticalsupportIntheexperiment37healthymaleSpragueDawley(SD)ratswererandomlydividedinto3groups：normalcontrolgroup(

7、C，n=8)，thecommonexercisegroup(CE，n210)andlongtimeexercisegroup(LE，n=lO)，eachgroupunifiedcommonfeedSwimmingmovementschemeforadaptive1week，notraining，groupCCEgroup60min，120minLEgroupwithoutweightloadingswimmingmovement，six

8、daysaweek，takeadayoffAftertheexperimentusingtheELISAmethoddeterminationoftestosterone(T)，3pHSD，P450c17，17pHSD；ELISAmethodforthedeterminationofserumtestosterone(T)，leptin(LEP)，F(xiàn)SH，LHlevels；Byrtpcrdetectionoftesticulartiss

9、ueofratsin3p—HSD，P450c17and17fIIHSDexpression；HEstainingtoobservemorphologicalchangesoftesticulartissueTheresult：1Testosterone3pHSDkeyenzymesinthetissueoftestisandCE，LEgroupcomparedwithCgroupweresignificantlylowerLEP450c

10、17contentgroupcomparedwithgroupC，wasextremelysignificantlylowerThecontentof1713HSDCE，LEgroupcomparedwithCgroupweresignificantlylowerThreekeyenzyme3pHSDmRNAexpressionofmRNA，P450c17mRNA，17pHSDmRNAcomparedwithgroupC，CEandLE

11、groupshavesignificantdifference2After10weeks’exercise，comparedwithgroupC，CEgroupratsTslightlylowerbuttherewasnosignificantdifferenceinserum，LEgroupisextremelysignificantdifference；ComparedwiththeCELEgrouparesignificantCo

12、mparedwithgroupCTintesticulartissue，CEgroupnosignificantdifference，LEgrouphavesignificantdifference3Serumleptin(LEP)，LH，F(xiàn)SH，comparedwiththecontrolgrouphadsignificantchanges，leptin(LEP)showedatrendofasignificantrise，andLH

13、，F(xiàn)SHhasdroppedsignificantlyBeforetherewasnosignificantdifferencebetweengroupsintheratweight，10weeksafterthemovement，twomovementgroupratsweightwereextremelysignificantdifferencecomparedwithcontrolgroupCEcomparedwithgroupC