基于Fe-,3-O-,4-磁性納米粒子新型免疫傳感器的研制.pdf

1、桂林工學(xué)院碩士學(xué)位論文基于FeO磁性納米粒子新型免疫傳感器的研制姓名：高會玲申請學(xué)位級別：碩士專業(yè)：分析化學(xué)指導(dǎo)教師：李建平20080401桂林工學(xué)院碩士學(xué)位論文AbstractFe304magneticnanoparticleswerepreparedusingcoprecipitationtechnologybythereactionbetweenFeS04andFeCl3。andFe300AUmagneticnanoparticl

2、eswerepreparedonthebasisofFe304，thenthesurfaceofthemagneticnanoparticleswerefunctionalizedbythedifferentmeansTheantibodywereimmobilizedonthesurfaceofthemagneticparticleswithabsorptionorcovalentbond。Theresultingmagneticbi

3、onanoparticleswithantibodywereattachedontothesurfaceofsolidparaffincarbonpasteelectrode(SPCE)withthehelpofapermanentmagnettoconstructnewimmunosensorsTheseimmunosensorspossesstheadvantagesofhighsensitivityeasytouseeasyreg

4、enerationetcThispaperhasdonesomestudiesaccordingtothefocusproblemsofelectrochemicalbio—sensorsatpresent，namelyhowtodevelopsimple，reliablemethodstoimmobilizebiomaterialsOnthesurfaceofsubstrateelectrode，andkeephi曲stability

5、andactivityofenzymeantigen，andantibody1Cysteine(Cys)moleculeswereirreversibleadsorbedonthenanoFe304surfaceThen，themodificationofsolidparaffincarbonpasteelectrodewithnanoFe304bychemicaladsorptionofglutaraldehyde(GA)wasach

6、ievedFinallygoatantihumanimmunogloblinG(antiIgG)werecovalentlyimmobilizedontheelectrodebyglutaraldehyde(GA)tomakearenewablepotentiometrieimmunosensorTheimmunosensorshowedaspecificresponsetohumanimmunogloblinG(IgG)inthera

7、ngeof0112nedmLwithadetectionlimitof0023ng／mLTheimmunosensorwasmadewitIlfacilitylow—costandusedexpedientlyTheimmunosensorbasedonmagneticpowderwasrenewableconveniently。Theimmunosensorwithbetterstabilityandhighersensitivity

8、Canbeusedextensivelyinbiomedicalandclinicaldetection2ThesurfaceofthemagneticFe304nanoparticlesWasfunctionalizedwith3一aminopropyltriethoxysilane(APS)toformaminomodifiedFe304Theanti—IgGWascovalentedtoaminomodifiedparticles

9、byusingglutaraldehydetoformmagneticbio—nanoparticlesTheresultingmagneticbionanoparticleswereattachedontothesurfaceofsolidparaffincarbonpasteelectrode(SPCE)withthehelpofapermanentmagnettoconstructanewamperometicimmunosens

10、orThisimmunosensorwasusedtoanalyzehumanimmunoglobulinGbydoubleantibodysandwichmethodThelinearrangewasfrom25to400ng／n!lLThedetectionlimitWas075ng／mL。Themagneticnanoparticlesofferedagoodmicroenvironmentforretainingthebioac