用胚挽救技術(shù)創(chuàng)制無(wú)核葡萄及三倍體新種質(zhì).pdf

1、本研究屬圍家高技術(shù)研究發(fā)展計(jì)劃(863計(jì)劃)(2001AA241172)、國(guó)家A然科學(xué)基金項(xiàng)目(30571280)、十一五科技支撐計(jì)劃(2006BAD0lAl702)和948項(xiàng)目f2005KZ40)資助的部分內(nèi)容，實(shí)驗(yàn)在農(nóng)業(yè)部西北園藝楠物種質(zhì)資源與遺傳改良重點(diǎn)開放實(shí)驗(yàn)室，部分實(shí)驗(yàn)在陜西農(nóng)業(yè)分r生物學(xué)重點(diǎn)實(shí)驗(yàn)室完成。西北農(nóng)林耕捉夫?qū)W碩士學(xué)位研究生學(xué)位論文用胚挽救技術(shù)創(chuàng)制無(wú)核葡萄及三倍體新種質(zhì)程琳琳論義評(píng)閱人：芻堡匹陳耀笪三飛菹塞監(jiān)王酉堊答

2、辯委員會(huì)主席：里堡廷提交論文日期：2QQ2生魚旦論文答辯LJ期：2QQ2生魚月!Q目學(xué)位授予日期：Y工兒33067DEVELOPⅧNTOFNOVELSEEDLESSAND田剛[PLOIDGRAPEV小mGEI岍LASNBYEⅣmRYESCUETECHNIQUEABSTRACTCrosscombinationsⅥ，eredesignedinwhichusedseedlessgrapecultivarsasmaternalparents4g

3、lOSScombinationsofseedlessxseededdiploidwcreRubyseedlessxXinyu，Dalihongseedlessx196，DelightxBeichunandn帕mpsonseedlessxXuefeng2grosscombinationsofseedlessxseededtetraploidwereEmeraldseedlessBlackolimpiaandEmeraldseedlessx

4、Fujiminofi4CROSScombinationofseedlesscuitivarselfpollinationwereDelightselfpollinationBlushseedlessselfpollinationDalihongseedlessselfpollinationandThompsonseedlessselfpollinationFirstofa11embryosWeIeculturedinvitrofor8w

5、eeksandthenculturedonseedling—formationmediumLater，glOSSprogeniesweretransplantedingreenhouseFurthermore，factorsofembryescueaboutovuledevelopmentmedium，ovuletreatmentsandcompatibilityofdifferentploidyparentswefcstudiedPl

6、oidiesofseedlessxseededtetraploidgrapeswereidentifiedwithFlowCytometryMainresultsWasasfollowing，1EmbryeVUetechniqueWasemployedand114seedlingsWereobtainedfrom10CROSScombinationsusingseedlessgrapecultivarsasmaternalparents

7、ThereWere75progenyseedlingsofseedlessserfpollination，15progenyseedlingsofseedlessxseededdiploid，24progenyseedlingsofseedlessxseededtetraploidSeedlingsobtainedinthisreseachwerediscrepantbecauseofdifferentgrosscombinationG

8、erminationpercentageandseedlingpercentageofThompsonseedlessselfpellinationwerethehighest40O％30O％respectively91seedlingsof8combinationsweretransplantedandpercentageofsurvivalseedlingsingreenhousewas90n％2Factorsofembryores

9、cuewerestudiedNitsehsolid1iquidmedium(supplement[exl005g，LC1)Wassuitableforovuledevelopmentandembryogenesis，whichusedDalihongseedlessandEmeraldseedless嬲maternalparentsItWasusefulforembryotogerminateandemergeseedlingswith

10、dissectedovulestreatment，butcuttingovulestransverselyCanbeusedifembryos伽’tbeseenwithnakedeyesinordertoavoidhurtingembryos11圮orderofparentalcompatibility，accordingtopercentagesofembryogerminationandemergingseedlings，Wasse

11、edlessdiploidselfpollinationseedlessdiploidxseededdiploidseedlessdiploidxseededtetraploidgrapes3Triploidcrossprogenieswereobtainedprimarilygtriploidand1tetmploidprogeniesofEmeraldseedlessxBlackolimpiaandEmeraldseedlessxF