陜西省蔬菜根結線蟲拮抗真菌的分離與篩選.pdf

1、分類號：墨壘圣蘭：壘UDC：632密級：坌五學校代碼：t0712研究生學號：SY20040175西北農林科教大學。2007屆攻讀碩士學位研究生學位(畢業(yè))論文陜西省蔬菜根結線蟲拮抗真菌的分離與篩選學科專業(yè)研究方向研究生指導教師完成時間中國陜西楊凌ISoLATINGANDSCREENINGOFANTAGONlSTICFUNGIoNVEGETABLERoOT_KNOTNEMAToDESINSHAANXIPROVINCEABSTRACTRoo

2、tknotnematodes(Meloidogynespp)areamajorgroupofpathogensofimportantcropsandcausegreatdamagetotheproductionofcropsEspeciallywinltheexpansionofcultivatedareaofgreenhousevegetable，rootknotnematodeshasbecomethebiggestobstacle

3、totheproductionofgreenhousevegetableandthebiologicalcontrolhasbeentakenmoreandmoreattentionrecently’Inthisstudy118vegetablerootsamplesinfectedbyrootknotnematodesWatecollectedfrom9countriescoveringYan’all，Weinan，Xi’anXian

4、yang，Yangling，Baoji，ShangluoofShaanxiProvince199antagonisticfungalstrainswgreobtainedbydirectdissectionrespectivelyfromfemales，eggmassesandeggsofrootknotnematodesTheseantagonisticfungiwalteidentifiedto22generaincludingFu

5、sarium，Cephalosporiopsis，Cladosporium，Alternaria，Paecilomycesereand43sterilefungiFusariumsppwasthedominantpopulationanditsisolationfrequencytookup34％ofalltheisolatesinthisstudyThespeciesandtheamountofantagonisticfungiofd

6、ifferentsamplesWeledistinctlydifferentinregionsThroughtestingtherelativepercentageofinhibitiontotheeggsofMincognitawiththesporesuspensionof131nonFusariumantagonisticfungiinvitro，Wefoundthatthemajorityofstrmnshadinhibited

7、theeggsof壇incognitaTherea增8strainssuppressedthehatchingofeggsOVer6096IsolateXPE36hashigherparasiticalabilitilytotheeggsotM，incognitawhichrelativepercentageofinhibitiontotheeggsofMincognitareached823％Theeffectoftheferment

8、ationliquidextractof68strainsFusariumspptosecondstagejuveniles(J2)of膨incognitaWastestedtoscreenthebiocontrolstrains9wefoundthatthemajorityofstrainshadnematicitalactivitydifferentlynerelativepercentageofdeathtotheJ2of膨inc

9、ognitaofLGEM5reached806‰andtherea∞7strainswhichrelativepercentageofdeathtotheJ2ofMincognitareached60～80％，withastrongnematicitalactivityInadditionthestrainLGEM5has900dthermalstabilitywhichfermentationliquidextractstillmai