版權(quán)說明:本文檔由用戶提供并上傳,收益歸屬內(nèi)容提供方,若內(nèi)容存在侵權(quán),請(qǐng)進(jìn)行舉報(bào)或認(rèn)領(lǐng)
文檔簡介
1、TableofContentsAbstract1中文摘要3Abbreviations5ChapterlBackgroundofKC’叻pgene711CharacterizationofKCTDIOandfamilymembers712StructureanalysisofKCTDl0913KCTDl0expressioninadulttissues1014TheregulationofKCTDl01115ThefunctionsofK
2、CTDl012Reference13Chapter2MouseKCTDIOcloningandexpressionpaRemduringtheearlyembryogenesis1721Introduction1722Materialsandmethods17221RNApreparationandreversetranscription17222Plasmidsconstructionl7223Embryopreparationfor
3、wholemountinsituhybridization18224Digoxigenin—UTPlabeledRNAprobespreparation18225Wholemountinsituhybridization1923Resultanddiscussion20231SequenceanalysisofmouseKCTDl020232KCTDl0expressionintheearlystagesofdevelopment20R
4、eference22Chapter3GenerationofKCTDl0conventionalknockoutmice23:;1Introduction23:;2Materialsandmethods23321Targetingvectordesignandconstruction23322PreparationoffeedercelllayersfromMEFcells24323EmbryonicstemfES)cellcultur
5、e24324ElectroporationoftargetingvectorDNAintoEScell25325IsolationofEScellcolonies26326FreezingEScellsin96wellplates26327ScreeningpositiveEScolonies27524Immunofluorescentstaining53J㈣辮525RNApreparation,semiquantitativereal
溫馨提示
- 1. 本站所有資源如無特殊說明,都需要本地電腦安裝OFFICE2007和PDF閱讀器。圖紙軟件為CAD,CAXA,PROE,UG,SolidWorks等.壓縮文件請(qǐng)下載最新的WinRAR軟件解壓。
- 2. 本站的文檔不包含任何第三方提供的附件圖紙等,如果需要附件,請(qǐng)聯(lián)系上傳者。文件的所有權(quán)益歸上傳用戶所有。
- 3. 本站RAR壓縮包中若帶圖紙,網(wǎng)頁內(nèi)容里面會(huì)有圖紙預(yù)覽,若沒有圖紙預(yù)覽就沒有圖紙。
- 4. 未經(jīng)權(quán)益所有人同意不得將文件中的內(nèi)容挪作商業(yè)或盈利用途。
- 5. 眾賞文庫僅提供信息存儲(chǔ)空間,僅對(duì)用戶上傳內(nèi)容的表現(xiàn)方式做保護(hù)處理,對(duì)用戶上傳分享的文檔內(nèi)容本身不做任何修改或編輯,并不能對(duì)任何下載內(nèi)容負(fù)責(zé)。
- 6. 下載文件中如有侵權(quán)或不適當(dāng)內(nèi)容,請(qǐng)與我們聯(lián)系,我們立即糾正。
- 7. 本站不保證下載資源的準(zhǔn)確性、安全性和完整性, 同時(shí)也不承擔(dān)用戶因使用這些下載資源對(duì)自己和他人造成任何形式的傷害或損失。
最新文檔
- 論毛姆的自由觀_29308.pdf
- GENE EXPRESSION ANALYSIS AND cDNA CLONING OF CHITINASE 5 AND 10 OF WHITE BACKED PLANTHOPPER,SOGATELLA FURCIFERA(HORVATH).pdf
- cloning and tissue expression of tnni3 gene in tianfu goat
- Expression of β-1,3-Glucanase gene in Escherichia coli.pdf
- The Modeling And Optimization Of Chemical Processes Using Gene Expression Programming.pdf
- Role of Trem-1 Gene in Immunity Against Inflammatory Diseases.pdf
- An Assessment of the Role of Multiple Gene Mutations on Phenotype Expression and Prognosis in Arvc Patients.pdf
- The DNA barcoding of CO I gene in some medicinal animals of Lacertilia.pdf
- Functional Analysis of Nicotiana sylvestris CBL Family Gene NsylCBL2.pdf
- Expession Diversity of Drought Resistance Gene Snac1 in Rice Germplasm.pdf
- 擬南芥RNA解旋酶突變體imprinted gene 1的表型分析.pdf
- the expressions of cd44v6 gene protein in breast cancer and its clinical significance
- Genetic Dixxection of Quantitative Trait in Yeast and Discovery of Single Feature Polymorphisms in Gene Expression Profiling Mi.pdf
- 29308.大連理工大學(xué)網(wǎng)絡(luò)教育校外學(xué)習(xí)中心管理模式研究
- 微陣列基因表達(dá)分析的臨床意義-gene expression profiling by microarrays clinical implications
- Gene Delivery into Mammalian cells by Recombinant Baculovirus and Antibody production of NS1 from Human Bocavirus.pdf
- Identification of Low Nitrogen Stress Induced Genes and Expression Analysis of the Thioredoxin Protein Gene Family in Rice(Oryz.pdf
- Low silicon rice gene 1調(diào)節(jié)水稻抗UV-B輻射的機(jī)制研究.pdf
- 基于網(wǎng)絡(luò)的肺腺癌coding gene和lncRNA的生物信息學(xué)分析.pdf
- Investigation into the Functional Significance of Precusor Messenger Rna Processing Factor 19(Prp19)gene Upregulation in Human.pdf
評(píng)論
0/150
提交評(píng)論