玉米花粉多糖對豬瘟弱毒苗免疫效果的影響研究.pdf

1、廣西大學碩士學位論文玉米花粉多糖對豬瘟弱毒苗免疫效果的影響研究姓名：溫納相申請學位級別：碩士專業(yè)：傳染病學與預防醫(yī)學指導教師：陸芹章2000.6.1StudiesoneffectofcornpollenpolysaccharidesforimmuneResponsetoAttenuatedHogcholeravirusChineseCstrainAbstract：Onthisstudyweusedalcoholprecipitation

2、toextractive3509ofcornposen，andacquired3889ofcrudeextractsAfterusedcrudeexffactdo(anthraone，molishseliwanoffiodide)reactionandhighpressureliquidchromatographtestidentificationcanvalidationthatit“SpolysaccharidesExperimen

3、talgroupswerevaccinatedwith①50ragpolysaccharidesHCLV；②100mgpolysaccharidesHCLV；③200mgpolysacchmidesHCLV；④physiologicalsolinesolutionHCLV；(eveygrouphavethreenonimmunepigs)everyweekabstractionrespectively；thendeteminecelli

4、mmunityandhumoralimmunitystandardTheresuRsshowed①inktest：antibodylevelofexperimentalgroupallhigherthancontrolgroupThoughttestif00s)significanceofdifference②TlymphocyteactiveErosettetest：Thepersentof們fiveErossettofexperim

5、entalgroupare：2184％2546％2192％allhigherthancontrolgroup1591％Throughttest(PO05)significanceofdefference；③Tlymphocytetransformationtest：thetransformationrateofexperimentalgroupare5532％，56390，5427％allhigherthancontrolgroup47

6、00％，andnotaddPHAgroup134％Throughttest口005)signigieanceofdefference；④Tlymphocytesubgroupassay：theexperimentalgroupsofavergevalueTlympocyteCD3、CIM、CD8allhigherthamthatofcontrolgroups，buttheNKcellofexperimentalgroupsandcont

7、rolgroupwerenotsignificanceofdifferenceThebestdossagewas100mg／pig，thenuSeittodofieldtrialtheantibodylevelwasmeasuredwithIHAtesttheresultswerecoincidentwithlaboratorytestThestudiesofresultdemonstratedthatcornpollenpolysac