巖藻聚糖硫酸酯酶產(chǎn)生菌篩選和酶學(xué)性質(zhì)的研究以及巖藻寡糖的應(yīng)用研究.pdf

1、中國(guó)海洋大學(xué)碩士學(xué)位論文巖藻聚糖硫酸酯酶產(chǎn)生菌篩選和酶學(xué)性質(zhì)的研究以及巖藻寡糖的應(yīng)用研究姓名：霍立華申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：水產(chǎn)品加工與貯藏指導(dǎo)教師：薛長(zhǎng)湖2003.6.1ScreeningoffucoidanaseproducingbacteriaandstudyonitsenzymecharactersandtheapplicationofoligosaccharidesAbstractFucoidansis硅uniqueclass

2、ofsulfatedpolysaeehalide娟丞manyspecialactivities。Andoligosacchaddesfromfucoidansarcmorevaluablethanthewholefueoidarmduetotheirlowconglufinationand確務(wù)solubilityInthispapersamplesalecollectedfromdifferentcoastalareasinQingda

3、oinordertoobtainbacteriaproducingfucoidanaseAfterenriching，separatingfirstscreeningandsecondscreening，lndueedmutationandscreeningafterinducedmutation，fivestrainscapableofproducinghigllfucoidanaseacGvities勰separatedandsel

4、ectedAmongthem。thes娃ainAhasthehighestactivityandthefucoidanaseactivityisashighas368UAfterthethalliandcolonyfeaturesaleanalyzedthesl：rainAisidentifiedtobeEosipetothrixRosenbachaccording幻Bergey’SManualofDeterminativeBacter

5、iologyBesides，theoptimalculturemedia，theoptimalfermentationconditionandsomefactorseffectsOnfucoidanaseactivityareinvestigatedthroughthesinglefactor船stBystudyingcharacteristicsoffucoidanase，aconclusionhasbeenmadethatthefu

6、coidanasehasahi酶thermalstabilityand拉stabilityAndfucoidanase鋤retainitsactivityUSder50centigradeandwithinpH47Atthesa艇letimethestudyshowsthat赫礦Canpromotethefucoidanaseactivitygreatlywhenitsconcentrationisuptol10一mol／1Inaddi

7、tion，theformationofcerium(IV)otigosaccharidecomplexesandeatal辯ichydrolysis&collagenbythecomplexesarealsodiscussedinthisresearchAfterfucoidansdegradingoligosaccharideswithdifferentmolecularwei#tF|，F(xiàn)2andF3，alegained。Themol

8、ecularweightrangeofFl，F(xiàn)2andF3alemorethan5000，from1000to5000andtessthant000respectivelyTheresultshowsthatF3isobviouslyeasytoformthecomplexwithceriumcomparedwithF2andF3Andtheeerium(IV)F3complexhasmuchgreatercatalyticactivi

9、tyofhydrolysiscollagenthanceriumOV)一Ftandcerium(IV)F2complexes，MoreovertheoptimumratioandtheoptimumhydrolysisconditionsarealsoobtainedinthisarticleKeyword：Fueoidan；Fueoidanase；strain；cerium(IV)oligosaccharldeeomplex；coll