具有漆酶作用內(nèi)生真菌的篩選分離及其特性研究.pdf

1、桂林理工大學(xué)碩士學(xué)位論文具有漆酶作用內(nèi)生真菌的篩選分離及其特性研究姓名：王玉兵申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：應(yīng)用化學(xué)指導(dǎo)教師：郝再彬201204ABSTRACTLaccase(EC11032)，atypeofpolyphenoloxidaseinvolvingmultiplecopperatoms，wasfoundbyJapaneseYoshidainLacquerin1883，andthennamedMetalcontainingoxida

2、sebyBertrangItwascapableofoxidizingmanykindsofaromaticcompounds，conversingphenolicsubstancesintonontoxiccompounds，anddegradingligninanditsderivatives，eliminatingthetoxicityinoilindustryInresentyears，laccasehasawiderangeo

3、fapplicationsinareassuchasenvironmentalpollutantsdegradationanddetoxification，pulpbiomanufactureandble孕ching，dyedecolorizationanddetoxification，degradationofligninanditsderivatives，transformationandfoodpropertiesmodifica

4、tion，eta1LaccasehasagoodecologicalandeconomicbenefitsTherebyithashightheoreticalandpracticalsignificanceinStudyonlaccaseanditslaccasestrainsThe154endophyticfungiwerefilteredoutfromSteviaplant，theperennialherbaceousplants

5、firstly，inwhich42，50，62plantendophyticfungiweregotfromleaves，stems，rootsrespectively，andthenidentifiedthateachspeciesofendophyticfungiarenotthesame。Afterthat154plantendophyticfungiwereinoculatedondetectionmediumcontainin

6、gtannicacid，guaiacoloranaphtholrespectively，andthethecolorofplatewasobservedAssociatedwiththeirgrowthspeed，15strainsofendophyticfungiwereselectedoutforfermenationandrepeatscreeningAfterfermentationofthe15plantendophyticf

7、ungi，itwasdeterminedthatthetimeofthehighestlevelofenzymeproductioninmoststrainswasnear10daftertheliquidfermentationbyABTSassayinlaccaseproductionprocessThreefungalendophyticfungi，namelyL02，L38，R28，havethestrongestLaccase

8、producingcapacityReviewofthethreestrainsthetimeofproducinglaccase，enzymeproductionandbiomass，L38wasconfirmedasaoptimalstrainforhighlaccasegenerationThroughresearchesforfermentationconditionsofL38，itwasdeterminedthatthebe

9、stearboflsourcewaspotatomixingcornpowder，nitrogensourcewasNH4C1，inorganicsaltionswereCuzandM92byusingsinglefactorsexperimentItwasexaminedthatthreekeyfactorsaffectingL38laccaseproductionweretemperature，NH4CI，andloadedliqu

10、idvolumeusedresponsesurfaceexperimentAfteroptimization，laccaseproductionmediumandthetrainingconditionswere：potato150∥Lcornpowder109／LNH4CI169Ig／LCuS045H20O059／LMgS04，029／LK2HP041嘰KCl059／LpHnature，andamountofliquid80mL／25