植物與微生物細胞壁糖的熒光輔助糖電泳分析.pdf

1、1J005057r，、#JtI℃辜卞斜筱大浮碩士學位論文植物與微生物細胞壁糖的熒光輔助糖電泳分析’；’化111L’J人‘利0qk卅甘投川J柞卅ff1[I】∞、唬；小物J，、學【m‘劉世曲救投20()iIr【】Jl2華中科技大學碩士學位論文AbstractSaccharidesaremajorstructureandfuctionalcompositionsoflivingthingsEspeciallyoligosaccharidesa

2、remajorparticipatorsinthelivingprocessSomeactiveoligosaccharidesarefromcellwallsofplantsandmicrobesToinvestigateoligosaccharides，weextractcellwallpolysaccharidesfromcellwallofSaccharomycescerevisiaeanddrygrazingbyalkalin

3、e—hydrolyzing，extractoligoxylanfromalkalinehydrolyzedgrazingbycrystaling，extractwatersolubilityextracellularpolysaccharidesbetweenkelpcellsbywaterdistilling，extractcellwallpolysaccharidesofSaccharomycescerevisiaeandwater

4、solubilityextracellularpolysaccharidcsfromkelpcellshydrolysatebyacid—hydrolyzingThenweanalyzethembythewayoffluorophore—assistedcarbohydrateelectrclphoresisSubsequentlNwecomparetheresultswithelectrophoreticchanoftheoligos

5、accharideadmixturefromacidhydrolyzedwatersolubilitystarchFluorophore—assistedcarhohs’drateelectrophoresis(FACE)isasimpleandinexpensivemethodforseparatingthecarbohydratesThe(01培o)saccharidesweretaggedwiththechargedfluorop

6、hore8aminonapbthalene一】，3，6一trisul^onate(ANTS)，andthereductiveaminationreactionswereessentiallycompleteafterapproximately16hrunderthegivenexperimentalConditionsSaccharide—ANTSadductswerethenseparatedfromoneanotherbyelect

7、rophoresisona32％CACR24％CBISpolyacrylamidegelatalkalinepHThistechniquedoesn’trequiresophisticatedinstrumentationandhighlytrainedpersonnelTheexperimentalresultsindicate：1，Theoligosaceharideadmixturefromacidhydrolyzedwaters

8、olubilitystarchCallformaseriesofbondsinelectrophoreticchanAndthefirstandsecondbondsarematchedtoglucoseandmaltose，separatelyItindicatesthatelectrophoreticchartoftheh，’dmlysateofwatersolubilitystarchcanshowthegradsdistribu