磁性納米顆粒固定化酶研究.pdf

1、華中科技大學(xué)碩士學(xué)位論文磁性納米顆粒固定化酶研究姓名：黃平英申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：趙元弟20110120II華中科中科技大學(xué)碩士學(xué)位論大學(xué)碩士學(xué)位論文AbstractEnzymeisakindofbiologicalmacromoleculeswithcatalyticabilityiswidelyfoundinnature.Catalysisbyenzymehasbeenprovedtoofferlot

2、sofspecialfeaturessuchasmildreactionconditions(biologicalpHtemperature)biodegradationhighcatalyticefficiencystrongspecificity.Duringthepastfewdecadesstudyontheimmobilizationtechnologyenzymecarriershasattractedalotofresea

3、rchers’attentionbecausethefreeenzymeisunstablecannotbereusedrecycled.Theprincipleofimmobilizedenzymeistoimmobilizetheenzymeontoaspecialcarriertokeepitinadefinedspacethroughsomeprocessestheenzymecanstillretainthecatalytic

4、ability.Magicnanoparticleshavebeenwidelyappliedinbiologymedicineenvironmentfieldsduetotheiruniquesuperparamagismgoodbiocompatibility.Enzymeimmobilizedontomagicnanoparticlescanbeseparatedfromthesolutionquicklyunderanexter

5、nalmagicfield.Anotheradvantageofmagicnanoparticlesasenzymecarriersistheirlargesurfaceareathuscanprovidemebindingsitesfenzymeimmobilization.Inthispaperenzymeimmobilizationonmagicnanoparticlesindifferentmethodswasinvestiga

6、ted.FthefirstmethodwatersolublemagicFe3O4nanoparticleswerefirstlysynthesizedbyacoprecipitationmethod.Thenlipasemagicnanoparticleswerecoentrappedincalciumalginatecapsulesseveralfactsaffectingtheenzymerecoveryactivitieswer

7、estudied.FthesecondmethodmagicFe3O4nanoparticlesweremodifiedwithaminosilaneinethanolacetonerespectivelytoproduceaminogroupsonthesurface.Thenalkalineproteasewascovalentlycoupledtotheseaminomodifiedmagicnanoparticles.Compa