30314.用于生物催化的磁性納米顆粒固定化酶研究

1、華中科技大學(xué)碩士學(xué)位論文用于生物催化的磁性納米顆粒固定化酶研究姓名：金欣申請學(xué)位級別：碩士專業(yè)：生物醫(yī)學(xué)工程指導(dǎo)教師：趙元弟20090519華中科技大學(xué)碩士學(xué)位論文AbstractIntherecentyearsalotofresearchesabouttheimmobilizingtechnologiescarriersofimmobilizedenzymehavedevelopedfast.Theprincipiumofimmobi

2、lizedenzymeistoimmobilizeenzymesontocarriersthroughphysicalchemicalmethods.Immobilizedenzymesmaintainnaturalcatalyticactivitycanbeseparatedeasilyfromreactionsolutiontoberecycledagain.Alargenumberofstudiesshowthatimmobili

3、zedenzymeshavebetterperfmancethanfreeenzymesinmanyrespects.Asakindofnovelcompositemagicnanoparticlesofferhighpotentialityfseveralbiomedicalapplicationssuchasdrugtargetingclinicaldiagnosistreatmentmagicseparationdetecting

4、carriersofimmobilizedenzymebasedontheirgoodbiocompatibilityuniquemagicproperty.Theimmobilizedenzymesusingmagicnanoparticlesascarrierscanbeseparatedfromtheproductbyexternalmagicfieldprovidingafeasiblewayfcontinuousproduct

5、ion.Suchmagiccarriersaregenerallyofceshellstructure:enzymesantibodiesnucleicacidsarecovalentcoupledtothemagiccethroughganicinganicshells.Theseshellscanbemodifiedwithvariousactivegroups(suchashydroxylaminosulphydrylsoon)b

6、ycopolymerizationchemicalmodification.Thesemagiccarriershavebeenwidelyusedfbiologicalmedicalresearch.Theimmobilizingmethodapplicationofimmobilizedproteaseontomagicnanoparticleswereinvestigatedinthispaper.Magitenanopartic

7、lesweresynthesizedbythermaldecompositioncoprecipitationrespectively.Comparingtheiradvantagesdisadvantagesthemagitenanoparticleswithanaveragediameterof25.4nmwerechosenasthecarrierssynthesizedbycoprecipitation.thentheywere

8、modifiedby(3aminopropl)triethoxysilane.Afterthatalkalineproteasewascovalentcoupledontothenanoparticlesusing14phenylenedllsothlocyanateascouplingagent.Furtherexperimentsshowedthattherelativeenzymeactivityofimmobilizedprot

9、easereached47.8%withcaseinservedassubstrate.thecatalyticactivityofimmobilizedproteasewasmestablethanfreeproteasewhichcouldretain98.632.37%after60days.Methan52%catalyticactivityremainedaftertheimmobilizedproteasehadbeenus