中國蛤蜊中樞神經(jīng)系統(tǒng)的解剖學研究.pdf

1、魯東大學碩士學位論文中國蛤蜊中樞神經(jīng)系統(tǒng)的解剖學研究姓名：張麗麗申請學位級別：碩士專業(yè)：動物學指導教師：王曉安20080607AbstractThenervoussystemoftheMactrachinensisiscomposedofapairofcerebralgangliapedalgangliaandvisceralgangliaThegeneralanatomicalcharacterandmicrostructurecha

2、racterofthenervoussystemoftheMactrachinensisWaSstainedbyHolmesBlestImpregnations，thentheresultsweredealedwithimageanalysissoftwareImageproplusThesizesofneuronsingangliacouldbedividedintothreeclassesaccordingtothearea“l(fā)ar

3、geneurons(3001J／n2)，intermediateneurons(100300tJa／12)andsmallneurons(100pxnz)Ingeneral。thedistributionofthesmallneuronsWasmorethanlargeneuronsandintermediateneuronsinganglia(cerebralganglia699％，visceralganglia803％，andped

4、alganglia697％)ThedistributionofthelargeneuronsWastheleastincerebralgangliavisceralgangliaandpedalganglia(cerebralganglia49％，visceralganglia5％，andpedalganglia38％)Theconclusionofthisstudywasthemicrostructurecharacterofthev

5、isceralgangliaWasthemostcomplexOnthecontrarythemicrostructurecharacterofthepedalgangliaWasthesimplestThesephenomenonsmightbedecidedbylivinghabitoftheMactrachinensisOnthisaccountthegeneralmorphologicstructureofnervoussyst

6、emreflectedtheadaptationtoenvironmentThepresenceinvestigationandmorphologyobservationofAchE(acetylcholinesterase)，5HT【5hydroxytryptamine)，DA(dopamine)，Glu(glutamate)，GABA“aminobutyricacid)，ACTH(adrenocorticotropichormone

7、)，SP(substanceP)，SOM(somatostatin)，NPY(NeuropeptideY)neuronsinthecentralnervoussystemoftheMactrachinensisweredonewiththeantiserumoftheninetransmitterbythemethodoftheSABC(spreptavidinbiotinperoxidasecomplexmethod)immunohi

8、stochemicaltechniqueTheresultsindicatethattheninetransmitters’nervecellsdistributedsymmetricallythroughoutthecerebralgangliaandvisceralgangliaAchE，5HTGluGABA，SPSOM，NPYnervecellsdistributedthroughoutthecentralnervoussyste

9、mACTHcellsweremerelydistributedinthecerebralgangliaandthereWasalittleofpositivefibersinthepedalgangliaDAcellsweremerelydistributedinthecerebralgangliaandnotfoundinthevisceralandpedalgangliaTheshapesofpositiveneuronswerer

10、oundellipse，irregularandSOonTheadrenalinandnoradrenalinwerewidelyexistenceinvertebratebutnotinMactrachinensisCNSBycontrasttheoctopamineandFMRFwerewidelyexistenceinmolluskbutnotfoundinvertebrateCNSPresumesometransmitterWa

11、sreserveininvertebrateandvertebrate，somewasreplacedwithotheranalogueandgeneratedsomenewtransmitterinvertebrateinthelastevolutionofnervoussystemTheresultssuggestedthatthedistributionandmorphologyoftheninetransmitternervec