肉蓯蓉（Cistanche deserticola Ma）種內變異性研究.pdf

1、Y湘Z2伽#女B：寧夏大學肉蓯蓉(CistancheEeserticolaMa)種內變異性研究Astudyonthegeneticvariationwithinspeeieeofcistanched￡oErl7icoioMa研究生：一是盅蘭二：指導教師：—_苤一垂監(jiān)班盎邑『11俯學位類剮：—型盟L——專業(yè)領域名稱：壘塹盟堂皇垃王生盤堂7研究方向；—j|!坐咝崖生僉王直垂L‘所在，學院：—生：金越堂堂睦二零零六年三月Astudyonthe

2、geneticvariationwithinspeciesofCistancheAbstractAbstract：Cistanehedeser療colaisbelongtothegrassofOrobanchacea，oftheparasiteplantofdesertandmedicineItishighmedicinalandeconomicvalueItanditshostplanthasbecomeanendangeredpla

3、ntduetodelugingfellanddug，increasingrequirementinthemarketanditsbiologicalcharacteristicsofparasiteItWaswrittendownChinaPlantRedDataBookAtpresent，scholarathomeandabroadhaveresearcheditspharmacologymedicineeffectandchemic

4、alcomportmentmensuratedandSOon，butthegeneticvariationwithinspeciesofCtstanchedeserficolaMahasnotstudiedWithinthewildCistanchedeserflcolaspecieswechoosethe9varimedtypesbased013thedifferencecolorofcorollalobesAnalysedofthe

5、13phenotypicquantitativecharactersofinflorescence，themaxCVis3404％，theminCVis904％，comparabilitycoefficientis0272708181andusetheUPGMAclusteranalysistodrawdendrngramAnalysedthe9varietedtypeswithRAPDmolecularmarkersWegropeth

6、ebestreactionconditousofRAPDPCRThe12pdmersusedinthisstudyamplified360scorableRAPDlociTheresultofanalysisshowsthatthepercentageofpolymorphiclociis3027％totalyanditislowerthanthespecieslevelWhileNei’Sgenediversityis01123and

7、Shannonsinformationindexis01633Thecoefficientgenedifferentiation(Gst)isO2323AnduseUPGMAclusteranalysistodrawthemolecularmarkerSdendrogramTheseindicatethatthereislowlevelofgeneticdiversityandthereare9variatedtypeswithinsp

8、eciesofC掃tanehedeserttcolaMawestudyonthegeneticvariationwithinspeciesofC括tanchedeserticolaM[a，whichcalloffertheimportanttheorytoknowtheresourcestructurewellprotectand黜itbetterKeywords：CistanehedesertieolaMavarioustypesUP