酪氨酸可逆蛋白磷酸化在植物細(xì)胞逆境信息傳遞中的作用.pdf_第1頁
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1、中國農(nóng)業(yè)大學(xué)博士學(xué)位論文酪氨酸可逆蛋白磷酸化在植物細(xì)胞逆境信息傳遞中的作用姓名:魏開發(fā)申請學(xué)位級別:博士專業(yè):果樹學(xué)指導(dǎo)教師:賈文鎖胡建芳20070601Abstractplantgrowthanddevelopmentarcinfluencedordisruptedinevitablybyenvironmentalstresses,plantshaveobtainedthemechanismandstrategiestoadaptor

2、resistantagainstexternalenvironmentalconditionsduringlongtimeevolutionCellsignalingtransductioniscriticalforsress_resistanccinPlantsandStresssignalingtransductionpathwayisverycomplexTheabscisicacid(ABA)’akeysignalingeomp

3、onent,regulationoftheoriginofABAsignalingplayimportantroleinstresssignalingpathwaysThisresearcholltheregulationmechanismofNCED3andAd03inABAbiosynthesisindicatereversibleproteintyrosinephosphorylationinvolvedinoriginofABA

4、signalingOurresearchhasfoundthatthekeyABAbiosynthesisgenesNCED3and4403expressedspeciallyinvascularbundletissuefurtherexperimentofsubcellularlocalizationthatNCED3andAA03localizedspecificlyinchloroplastandcytoplasmcorrespo

5、ndinglyshowthatthetransmembranetransportofprecursormalghtmanipulatetheoriginofABAsignalingAsNCED3andAA03couldbeinducedsignificantlybydroughtstress,WiththehelpoftransgeniesystemoftightlycontrolledandhigIllyinducibleXVEexp

6、ressionsystem,increasingNCED3expressionupregulateAd03transcriptionallevel,theseobservationssuggestNCED3isinvolvedinapositivefeedbackregulationofAA03geneexpressionunderdroughtstressWefmdadublefunctionalkinaseofMAPKKKfamil

7、ynamedasMAPKKK6(42)inlargescaleArabidopsismutantscreeningThemutantofMAPKKK6(42)leadstolowertranscriptionallevelof4A03studiesshowthatitlocalizedspecificlyinthemembranechemicalinduciblesystemsidentifiedthatincreasingexpres

8、sionofM4egrac6(42)upregnlatetheexpressionofAdOJgenethefindingssugestthatMAPKKK6(42)mightplayimportantrolesintheregulationofABAsignalingaccumulationExperimentsofpharmacologyindicatethatPTPaSehavearelationshipwithoriginofA

9、BAsignalingassp∞incal|yinhibitorofPTPasccanefficientlyinhibitedABAaccumulationZmRIDPIamemberof]rrp目kqefamilywasfirstclonedandputtiedfrommaizebourlab,theexpressionofZmRIDPIupregulatcobviouslytheproductionofAA03Mmatheresul

10、tmeansP1ma∞involvedintheABAsignalaccumulationAntotherPTPasewasfoundinArabidopsisnamedasPTPl35themntuant醴PTPl35leadstolowertranscriptionalevelofdroughtinduciblegeneLTl30TheganeexpressionanalysisshowedthatinoverexpressionP

11、TPl35plants,upregnlatetheexpressionofTUBlmRNA,downregulatetheexpressionofCOFTCHSREDl。PIP4。GI。HRBIPIP3CR88ELF3,havelittleinfluence∞RBC$M,工觀CCAIWeidentifiedthatP1Pa∞notonlytakepartinthestressesmessengertransportbutalsoinvo

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