大團囊蟲草菌的發(fā)酵工藝優(yōu)化及藥效成分分析.pdf

1、浙江大學(xué)碩士學(xué)位論文大團囊蟲草菌的發(fā)酵工藝優(yōu)化及藥效成分分析姓名：陳少云申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：李永泉20070601浙江人學(xué)碩十學(xué)位論文AbstractCordycepsophioglossoides，arareandvaluedtraditionalChinesemedicine，belongstoCordycepsClavicipticeaeHypocrealesAscmycotinaAccordin

2、gtotherecordationof“Xinhuabencaogaiyao”，thismedicinalfungusisusedtocureMenoxeniaandcanenhancetheimmunityHoweverCordycepsophioglossoidesasanaturalresourceisnowclosingtodieoutduetoenvironmentdeterioration，SOartificialculti

3、vationisaneffectivemethodtetrievethisorganismInthisWOrktheliquidcultureconditionsofCOphioglossoidesfortheproductionofmyeelialbiomasswereinvestigatedin15LagitatitionfermentorandtheactivecomponentsofCophioglossoidesmycelia

4、wereanalyzedThestrainCophioglossoidesLi01whichwasisolatedfromthewildfruitingbodyofthefungusinYurmamThebasicculturemediumwas669／lofsucrose，10gnofyeastextract，30％(v／v)ofsilkwormhydrolysate，049／lofMgS04。7H20and04鯽ofKH2P04Fo

5、roptimizingfermentationcondition，theorthogonallayoutL9(4’)Wascarriedoutin15LagitatitionfermentorundertheconditionsoftheprimaryaerationrateasO6vvmandagitationrateas150rpminwhichthefourmaininfluencefhctorswereselectedastes

6、tfhctorsincludinginoculumvolumtemperatureinitialpH，metaphaseaerationrateTheexperimentalresultsindicatedthattheoptimumfermentationconditionswas5％ofinoculumvolumtemperature23℃pH55andaerationratechangedtolwmafter40hUnderthe

7、optimumfermentationconditionsabove，the959nofmaxiummycelialbiomasswasachieved，whichisabout2timeshigherthanthatatthebasicfermentationconditionsInadditiontheanalysingmethodoftheactivecomponentswereestablishedforCophioglosso

8、idesmyceliaandtheresultWasrevealedtobe1406mg／gofpolysaccharides，5889mg／gofmannitol、7479m∥gofproteins、217m∥gofadenosineundertheoptimalcultureconditionsKeywords：Cordycepsophioglossoides；optimizationoffermentationprocess；an