石斛化控的生理生化研究.pdf

1、西南農(nóng)業(yè)大學(xué)碩士學(xué)位論文石斛化控的生理生化研究姓名：夏鴻西申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物化學(xué)指導(dǎo)教師：朱利泉張玉進(jìn)20000501STUDIESONPHYSIOLOGYANDBLoCHEMISTRYoFD匹^∞假D酣聊ⅣD露兒ETREATEDWITHPLANTGRoWTHREGULAToRSHongxixia(Instituteoff咖ScienceSouthwestAgriculturalUniversityChongqing40071

2、6)Dendrobiumplantseedsareassmallasdust，anditsgeminationrateisnotmorethan5％EvenmorebecausewiidbendrobiumplantgrowtheonditionisstricttherateofeffectivetilleringandtheoutputwereextremlylowThat1imitedthedevelopmentofDendrobi

3、umindustryInordertoincreasetheoutput，weimmersedtherootsof口nobileindifferentPlantGrowthRegulators(PGRs)twohoursbeforehightilleringperiodandwatertherootwithdifferentratioofPGRsinhighgrouthperiodThedynamicvariablerulesofint

4、ernalhormoneswerealsostudiedwithHPLCmethodtoprovideguidefortheintroductionandcultivationofwildDendrobiumP1antThedetailedtreatmentexperimentsarecarriedoutin1999withGA』and6BAaccordingtotheresultoflastyearselectionfromGA，、6

5、一BA、NAAandPPmThestatisticsresultshowsthatPGRscanincreasenotonlYtheactivityofPODandSOD，butalsothecontentofsoluahleproteinandsugar：GAland(or)5BAinducedyieldingthenewPODisoenzymePI、R、P；、P：andnewSODisoenzymeg、S3Theproteingel

6、gramsaredifferentwithdifferenttreatmentandatdifferenttimeWeusedBromocresolcolormetrytomeasuretheeffectofGA1and6BAtreatmentonalkaloldItwasfoundthatalkaloidincrease67101％and34％whentreatedwith05lmg／LGAandlOmg／k6BATLCanalysi

7、sshowsthatthecontentofdendrobineisnotaffectedProabelYotherkindsofalkaloidaccumulatedmoreornewoneappearMeanwhiletheoutputincrease133307％and173400％treatedwith05lmg／LGAaand10—50mg／L6BATherateofeffectivetilleringincreasedto6