楊樹根癌病拮抗菌的篩選及其拮抗活性的初步研究.pdf

1、河北農業(yè)大學碩士學位論文楊樹根癌病拮抗菌的篩選及其拮抗活性的初步研究姓名：梁靜申請學位級別：碩士專業(yè)：森林保護指導教師：崔建洲袁玉欣20070610ScreeningofAntagonisticStreptomycesSWainsandthoseInhibitionActivitiesagainstAgrobacteriumTumefaciensSpeciali夠ForestprotectionDh酬∞ofstudy：Biocontro

2、iofforestpathologyPostgraduate“L1ANGJingsl珥日慨CUIJimlidlouYUANYuxinHUANGDazheangAbstasctInthiseⅨperiment，thepathogenofpoplarCrowngallwereisolatedandidentifiedBesidesthatbaaeri皿epiphyte齜ptomyeesWCfeusedto8crⅫbiologicalcont

3、rolagentsagainstAgrobacteriummmefaciens，andthephysicochemicalcharacteroftheantibioticsubstancewasanalysed，whichwilllaythegroundworkforbioconuolofthediseasebyotherantagonisticmainsinthefutureFinallytheinhibitedroleofsomeb

4、scteriocidesandantibioties011lgrobaeter／umtumefaciensscreenedfrompoplaranditsinhibitoryisolateswantestedtoguidingchemicalcontrolofpoplarcrowngallandtherequixcmentofdevelopingrootedbiologypesticideInthetestelevenisolates、

5、“血theconfigurationcharacterofAgrobacter／umtwnefac／enswereobtainedfromthepoplarrootandallaccordedwiththecharacteristicoflgrobacter／ummmefac／ensbybcingobservedOnmicroscopeByinfectingoncarrotandpoplaronlythreeisolatescauocd

6、themtocomeforthgallSothethreeisolateswereidentifiedtobepathogencausingpoplarcrowngallTwentythreestrainsofbscterlaelevenstlainsoffimgi，sixtythreestrainsofStreptomyeesisolatedfromtwotypepoplarrhizospherein血isstudy_K∞tested

7、forantibioticisolatesagailLstpathogeneticisolateA2frompoplarorOWllgallEightisolates267％inanthenumbersoftestedbact耐ashowed柚antibiosisactivityThereintotwoisolatesshowedahigherantibiosisactivitywilhadiameteroftheinhibitionz

8、oneragingfrom!530cmsixofthemshowedalowactivitywithadimueteroftheininbitionzonelowerthanl5cnWhilealltheelevenSUainSoffungishowednOanu“binsisactivityOndoublelayeragarsixtythreestrainsofStreptomyce8isolatedfromtwotypepoplar

9、thizosphereweretestedforantibioticisolatesagainstpathogenicstrainA2andseventermisolatesshowedmantibinsisactivitythemteofinhibitoryisolatesWan269FiveSWeptomyeeastmi眠295％inallthembibitoryisolatesshowedastrongantibiosisacti

10、vityagainstpathogenicstrainA2withtheinhibitiongolleabove30oreThestainnamedTS22showedthestrongestantibiosisactivityamongthemwiIh380cmofzoneinhibitionespeciallyBesidesthatfivestrainsofStreptomyceskcptinourlabweffetestedfor

11、antibioticisolatesagainstpathogenicsUainA2，andthreeisolatesshowedahigherentibinsisactivitywithadiameteroftheinhibitionzonerangingfrom153。0cnTheoptimumfermentationconditionsweredeterminedthat25mlliquidsoybeancakemedium(mc

12、dimB)w∞addedin250mlflaskandW91eculturedat28℃，pH70，180r／rainfor72hTheantibioticsubstanceproducedbyTS22showedastableantibioticactivitytoheatandpHThissubstancenotonlyexistedinthefermentationliquidbutalsointhemycelinmitWanso