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1、UniversityCode：10225RegisterCode：S15748DissertationfortheDegreeofMasterBiocontrolandMolecularDiagnosisOfFusariumsolaniCandidate：Supervisor：AcademicDegreeAppliedfor：Speciality：Dateof0ralExamination：University：ShaYilaPaNax

2、iLiuXuefengprofMasterForestProtectionJune2015NortheastForestryUniversityAbstractTheelmisanimportantgreeningtreespeciesandafforestationspeciesinnorthemchinaandplaysahugeecologicalroleinsand—fixationanderosionpreventionInr

3、ecentyears，greateconomic10ssesandecologicaldamageshavebeencausedbythebandedelmbarkbeetleTherearenoefiectireandenvironmentallyharmoniousmethodstocontrolitasyetTherefore，lookingforthenonpollutionmeasurestopreventandcontrol

4、theplantdiseases，screeninganddevelopingnewmicrobialfungicidesforFusariumsolaniisveryimportantInthisstudywecollectedthediseasedelmsattaekedbyScolytUSschevyrewiinXinjiangUrumqiandHeilongjiangHarbinandattemptedtoisolateanta

5、gonisticmicrobethatCancontainFJDla船iandstudyonitsidentification，antimicrobialactivityoffermentationbrothandoptimizationofitsfermentationconditionsforoptimalinhibitoryeffectsInaddition，withthesDecificprimersdesigned，throu

6、ghthenestedPCRamplificationestablishedasetofEsolaniisfastdetectiontechnology1Fromthecollectedelms，microbeswereisolatedusingthetraditionalmicroorganismis01ationmethodsEsotaniwasusedasplantpathogentotesttheresistanceabilit

7、yoftheisolatedmicroorganismwithslidecultivationmethod，amongwhichthreestrainsshowedinterestedfeaturesFermentationbrothfiltraterescreeningresistanceabilityscreenlngeventuallyidentifiedoneoptimum，JN1，fromthethreescreenedstr

8、ainsJN1mostlyretardedthegrowthofEsolanimoldwiththerespectivesuppressingratesas534％2Combinedwithrelatedpapers，theevidencefromtheculturalperformances，morphologicalcharacteristics，physiologicalandbiochemicalfeaturesandthese

9、quencingandanalysisofits16SrDNAofJN1strain，weinitiallydefineJN1isBacillusmethylotrophicus3FermentationconditionoptimizationwascarriedoutusingsinglefactorandorthogonaltestsTheresuitsshowedthatthebasicculturemediumforJNlis

10、，anditgrowswellwithinitialpHwas70，fermentationtimewas54h，cultivationtemperaturewas28。C，androtatespeedwas180r／min4BasedondifferencesinITSsequencesofEsolaniandotherdominantspeciesoffungiassoeiatedwithSschevyrewiapairofspec

11、ies—specificprimers，F(xiàn)sal／Fsa2wassynthesizedTheITS—PCRmoleculardetectionforthetestedstrainshavedetectedbyspecificprimersFsal／Fsa2Theresultsshowedthat，DNAextractedfromFusariumsolanistrainprovidedasingle500bpamplicon，butDNA

12、extractedfromotherstainsdidnotamplifyusingtheseprimersInthestudythesensitivityofspecificprimersFsal／Fsa2hadexamined，indicatingthattheDrimerssensitivityforgenomicDNAdetectionisonthelevelof1fg／tLKeywordsTheelm，F(xiàn)usariumsola

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