Co（Ⅱ）、 La（Ⅲ）與HSA或BSA的結合平衡研究.pdf

1、廣西師范大學碩士學位論文Co（Ⅱ）、La（Ⅲ）與HSA或BSA的結合平衡研究姓名：邊賀東申請學位級別：碩士專業(yè)：有機化學指導教師：梁宏20000101BindingEquilibriumStudyBetweellc“Ⅱ)，La(IⅡ)andlISAorBSAPsotgradulate：BianHe。DongSupervisoEProfessorLiangI0ⅡgAbstractThehindillgofco(11)andLa(1]1)t

2、ohanlanSelLimalbumin叫SA)Orbovineselumalbumin(BSA)andthec㈣pec日onwithcu(IIX舀《Ⅱ)orCa(Ⅱ)havebeenstudiedbyequilibriumdialysisatphysiologicalpH(743)andpH(63以respectivelyTheScatehardanalysisindicateslhattherearethreeslrongbindi

3、ngsites12and15weakbindingsitesofC0fII)inHSAandBSArespectively；andtheream2strongbindingsites，8and6weakbindingsitesofLa(m)inHSAandBSArespectivelyThesuccessivestabilityconstantsofCo(II)一HSA，Co(II)一BSA，La(m)HSAandLa(III)_BSA

4、areobtainedbynonlinearleastsquaremethod溉BjerrumformulaTheorderofmagnitudeofklisfoundtobelOThesuccessivestabilit3const2al佶ofCo(II)一眥and叫Ⅲ)HSAm鐘柵衄l衄ofCo(II)一BsAandLa(mFBSATheresultsofthecompectitionbetweenC0(II)andCu(II)，z

5、n(II)orCa(II)indicatethatoBeofthestrongbindingsitesofco(II)inHSAofBSAismostprobablylocatedattheIripeptidesegmentofNterminalsequenceofHSAandBSAtakingoctabedralconfigurationAnotherbindingsiteislocatedinsideoftheproteinntco

6、ordinatedatomsaretwoOrthreeNitrogena幻msofimidazoleandoneortwooxygenatomsofcarhoxy1thattaketetrahedralconfigurationThelastoneiscoordinatedwithalmos【alIoxygenatomsJudgedfromthecompectitionbetweenLa(Ⅲ)andCu(II)，Zn(II)orCd(I

7、I)，OneofthestrongbindingsitesofLa(m)inHSAorBSAismostprobablycoordinatedwithalmostalloxygenatomsTheanalysesofHillplotsandfreeenergycouplingshowflaatthebindingofCO(II)toHSAandBSAresultssomepositivecooperativeeffect(C0(II)H

8、SAsystem^產(chǎn)27；co(1I)BSA，^旺14Xandtheformerisgre塒erthanthelatterInⅦⅢ瑚SAandLa(m)BSAsystemstherearesomenegativecooperativeeffect(La(III)HSAsystem九i=o83andLa(m)BSA，^—卸88)KeywordsCo(II)albumin，La(III)albumin，equilibriumdialysis