細粒棘球蚴鐵蛋白基因的重組表達、免疫學特性鑒定及免疫保護力研究.pdf

1、寧夏醫(yī)科大學碩士學位論文細粒棘球蚴鐵蛋白基因的重組表達、免疫學特性鑒定及免疫保護力研究姓名：丁聃申請學位級別：碩士專業(yè)：遺傳學指導教師：趙巍王婭娜20090401寧夏醫(yī)學院碩士研究生學位論文英文摘要RecombinantexpressionidentificationimmunologicalactersimmunologicalProtectionofferritingenepET28afromEchinococcusGranulos

2、usABSTRACTObjectToconstructtherecombinantexpressionplasofEchinococcusgranulosusfeitininChinesemainlstrainexpressingthepurifyingrecombinedferritin.Tostudytheimmunologicalacteristicsimmunologicalprotectionofrecombinedferri

3、tintosupplyanewcidatemoleculefthemanufactureofmoleculevaccineinechinococcosis.Method(1)Comstructionexpressionimmunologicalactersticsidentifyingoftherecombinantexpresiionplas:①Clonethetargetgenetoexpressionvacterofthentra

4、nsfmingittopET28ainduceitexpressingrEg.ferritintherEg.ferritincanbepurefyedwithHisbindresincolumwhichincludingNi2②UsetheantiserumofimmunemicewithrEg.ferritin，tostudytheimmunologicalacteristicsthroughWesternblotELISAofrEg

5、.ferritin(2)ThestudyofimmunologicalprotectioninrEg.ferritin:attacttheimmunedmicewhichgotrEg.ferritininfectedbytheprotoscolexofEchinococcusgranulosusfromabdominalcavity.Result(1)AgeneenginerringbacteriumrEg.ferritinpET28a

6、BL21(DE3)plysShasbeenconstructedrEg.ferritinhasbeenexpressedpured.Westernblotdetection:rEg.ferritincanbedistinguishedbytheantiserumofimmunedmicenaturalantigen(protoscolex)thelocationwasassameas19KD.ELISAshowd:IgGintheimm