母血胎兒DNA與子癇前期關(guān)系的研究.pdf

1、復旦大學碩士學位論文Y77307工母血胎兒DNA與子癇前期關(guān)系的研究培養(yǎng)單位：上海市第一婦嬰保健院專業(yè)：臨床婦產(chǎn)科學姓名：劉銘指導教師：段濤教授導師組成員：肖君華老師蔣躍明老師二零零五年五月秉野弘考鼻露幫扔皇支0；誰QuantitativeAnalysisofFetalDNAinMaternalPlasma：ImplicationsForAnIncI屯asedriskofPreeclampsiaobiective：Toobservewh

2、ethertheabnormalincreaseofcirculatingfetalDNAconcentrationispresentbeforetheclinicalmanifestationofpreeclampsia；TostudyifthiscouldbepredictiveofthedevelopmentofpreeclapsiaMethod：Wecollected5mlofmaternalbloodfrompregnantw

3、omenattendingforafirstantenatalvisitCaseswereasymptomaticwomanwhosubsequentlydevelopedpreeclampsiamatchedtocontrolwomenforparityandgestationalageDNAwasextractedfromplasmasamplesbyuseofaQIAampBloodKit(Qiagen)，andreal—time

4、quantitativepolymerasechainreaction(PCR)quantifiedfetalDNAusingTaqManprimersandprobesdirectedagainstSRYgenesequencesinthematernalcirculationResult：Thereare17casesofwomenwhosubsequentlydevelopedpreeclampsiaand34controlwom

5、enMeangestation(SD)oftwocaseswasl8230WeeksThemedianfetalDNA1evelinthecasewassixtimesofthecontrol’SConclusion：FetalDNAincreasesinthematemalcirculationinpregnancywomenwhosubsequentlydeveloppreeclampsia，andthereappearstobea