胃癌中OPRT和DPD mRNA表達(dá)的檢測與臨床意義.pdf

1、內(nèi)蒙古醫(yī)學(xué)院碩士學(xué)位論文胃癌中OPRT和DPDmRNA表達(dá)的檢測與臨床意義姓名：張東明申請學(xué)位級別：碩士專業(yè)：外科學(xué)指導(dǎo)教師：董培德20090501。內(nèi)蒙古醫(yī)學(xué)院碩士研究生學(xué)位論文(2009年)ExpressionofOPRT，DPDmRNAingastriccanceranditsclinicalsignificanceAbstractObjectiveThepurposeofthisstudywastoinvestigatether

2、elationshipbetweenthelevelsofrnRNAof5一FUmetabolicenzymesandclinicopathologicfactorsingastriccancerMethods53gastriccancersampleswithpathologicalconfirmedwerecollectedfromthedep—artrnentofgeneralsurgeryoftheaffilatedhospit

3、alofinnermongoliamedicalcollegeWemeasuredthelevelsofOPRTandDPDmRNAinpairedgastriccancertissueandsurroundingnormaltissueWealsoinvestigatedthecorrelationsbetweenthemRNAlevelsofthesetwoenzymesandclinicopathologicfactors(sex

4、，age，tumorsize，histologicaltype，depthoftumorinvasion，extentoflymphnodemetastasisandpTNMstage)ResultsTherelativeexpres—sionvalueofOPRT～mRNAingastriccancertissue(115056)washigherthanthatofthepairednormaltissue(088031)Theex

5、pressiondifferencebetweenthetwogroupsWaSsignificantstatistically(t=366，P0。05)；BoththeOPRT—mRNAandtheDPD—mRNAexpressionintumorsshowed8positivecorrelationwithoneofpairednormaltissues，OPRT—mRNA(r=028，p=0049)，DPD—mRNA(r=037，

6、p=0020)，respectivelyTherel—ativeexpressionvalueofOPRT——mRNAtendtObelowerincaseswithlymph——nodemetas—tasis(100052)thanincaseswithoutlymph—nodemetastasis(142054)，(P=0007)；expressionofDPD—mRNAinmucinousadenocarcinoma，Poorly

7、differentiatedadeno—carcinoma，moderatelydifferentiatedorwell—differentiatedadenocarcinomaweresignificantstatistically(F=2771，P=0000)Poorlydifferentiatedadenocarcinomaandmucinousade—nocarcinomashowedasignificantlyhigherDP

8、I)——mRNAthandidmoderatelydifferentiatedorwell—differ—entiatedadenocarcinoma，thehighestexpressionDPD—mRNAwasfoundinmuci—nousadenocarcinomaConclusion1TheexpressionofOPRTmRN：＼ingastriccar—cinomatissuewashigherthanthatinthep