化學發(fā)光法檢測焦磷酸及功能化納米四氧化三鐵的制備.pdf

1、河北大學碩士學位論文化學發(fā)光法檢測焦磷酸及功能化納米四氧化三鐵的制備姓名：呂學沖申請學位級別：碩士專業(yè)：分析化學指導教師：李正平20090601AbstractAbstractPyrophosphateistheintermediateinmanykindsofmetabolicreactionanditplaysanimportantroleinlifeprocessIndailylife，pyrophosphateismainlyu

2、sedaswatersofienertandfoodadditivesTherefore，theanalysisofpyrophosphateisveryimportantThedetectionofimmunoassayDNAhybridizationandSNP(singlenucleotidepolymorphism)analysisplaysaveryimportantroleinlifesciencesTheyhavebeen

3、widelyusedinbiochemistrymedicaldiagnosticsAftertheradioactiveassayandenzymaticassayamethodofchemiluminescenceusingmetalnanoparticlesasprobeshasbeenrevealedBydetectingthemetalionsfrommetalnanoparticles，wequantitativedetec

4、ttheimmunoassayDNAhybridizationandSNPanalysisandtheresultissatisfactied、撕thhighsensitivityBecausetheusualmetalnanoparticlesasAu，Agarehardtobedissolvedintometalions，themetallicoxide—Fe304easilydissolvedhasbeenpreparedandt

5、heresultsofFe計，F(xiàn)e2十havegooddeedinchemiluminescenceItprovidesanewwaytoimprovethesensitivitythroughthemagnetismofFe304whichCanseparatesandpreconcentratesthetestedcompositionAsimpleandeconomicalmethodforsensitivedeterminati

6、onofPPi(pyrophosphate)hasbeenestablishedandsuccessfullyappliedtodeterminepyrophosphateacidinrealsampleWealsopreparedthenano—particlesofFe304witllnosurface—modifiedanddetectedthemolecularofFe304usingthemethodofchemilumine

7、scenceThelimit(30，n11)is3OxlO叫mol／L；TheFe304ishardtobeusedinanalysisofbiochemicaltestforthepoorwatersolubilitySo，wepreparedamine(carboxyl)functionalizedmagnetitenanoparticlesWith16一hexanediamine(polyacrylicacid)asamine(c

8、arboxyl)funcfionalizationreagentTheamine(carboxyl)notonlyimprovesthewatersolubilitybutslaoprovidesthepossibilitytocouplethebiologicalmoleculesThefunctionalFe304Canbeusedasdoublefunctionsprobe(detection，separationandpreco