SPR傳感器的研制及其對偶氮苯-環(huán)糊精相互作用的研究.pdf

1、南京航空航天大學(xué)碩士學(xué)位論文SPR傳感器的研制及其對偶氮苯環(huán)糊精相互作用的研究姓名：陳佳棋申請學(xué)位級別：碩士專業(yè)：應(yīng)用化學(xué)指導(dǎo)教師：張海黔20070301SPR傳感器及其對偶氮苯環(huán)糊精相互作用的研究iiABSTRACTSurfacePlasmonResonance(SPR)technologyanewsensingtechniquewasfirslyreptedin1980sinvolvingtheknowledgesofopticsc

2、hemistryelectronicsetc.Thetechniquepossessesmanymeritssuchasrealtimeswiftlyresponsehighsensitivityunpollutionlabelfreeneedlesssamplejustbecauseofwhichithasbeenbecomingaleadingtechniquefbiomolecularinteractionanalysesduri

3、ngthepasttwodecades.Inparticularthetechniquehasbeendevelopingtremendouslysinceitsapplicationinthelifesciencesinthedleof80s.NowdaystheapplicationofthesenssbasedonSPRtechnologyhavebeenfoundinavarietyoffieldssuchasopticsmed

4、icinepharmaceuticsenvironmentologysooninmanycountries(USAJapanherlsSwedenetc).Themainwksinthisthesisare(1)todesignconstructanintegrateddifferentialSPRsenssystem.asystemtovalidatethefeasibilityofthisdifferentialSPRsens.Ba

5、sedontheprinciplesofSPRwecompiledaprograminLabVIEWlanguagetosimulatenumericallytheconventionalSPRresponsecurvesobtainedoptimalparametersfSPRsens’sdesigndebug.WehavepreparedboththehardwaresoftwareofSPRsensconstructingthes

6、ensingsystem.TestingthedependenceoftheresonanceangleshiftontheconcentrationofethanolshowsthattheSPRsenswksverywell.(2)toemploytheSPRsensconstructedbyourowntostudytheinteractionbetweenβcyclodextrinazobenzene.Firstlywesynt

7、hesizedthiolatedβcyclodextrinfthepurposeofselfassemblymonolayeronsurfaceofgoldfilm.Nexttheinclusioninteractionbetweenβcyclodextrinazobenzenewasstudiedwithspectroscopyelectrochemistry(SquareWaveVoltammetrySWV)offeringther

8、esultswhichwouldbeusedfcomparisonwithSPRresult.FinallyobservationontheinteractionbetweenβcyclodextrinazobenzenewascarriedoutbyusingSPRsens，indicatingthattheresultsbySPRareingoodagreementwiththatbyspectroscopyelectrochemi