氫飽和水對大鼠實驗性牙周炎抗炎作用的初步觀察.pdf

1、青島大學碩士學位論文氫飽和水對大鼠實驗性牙周炎抗炎作用的初步觀察姓名：孟祥嬌申請學位級別：碩士專業(yè)：口腔基礎醫(yī)學（牙周病學）指導教師：鄧婧；袁昌青20120519Abstract川IlllIIIllIIIllllIIIIIY2088140Objective：Chronicperiodontitisisanon—specificinflammationinitiatedbydentalplaquemicroorganismswhichoc

2、curresinperiodontaltissuesModempathogenesisofperiodontitisstudiessuggestthatmostofthetissuedamageisduetothesystemicoverreactiontothepathogensAconsiderablenumberofexperimentsshowthatoxidativestressisanimportantpathogenicm

3、echanisminperiodontitisRecentstudieshavefoundthathydrogenisanovelbiologicalantioxidantswhichhavetherapeuticeffectsonavarietyofdiseasesrelatedtooxidativestress，andhavetheabilitytoslowdownperiodontitisprogressThisStudyobse

4、rvedtheperiodontalinflammationreactioninratsdrinkinghydrogenrichwaterandpreliminarilyinvestigatedtheeffectofhydrogenoninfectiousbonediseaseMethods：24maleSprague—Dawleyrats(8weeksofage)wererandomlydividedinto3groups：(1)N(

5、thenormalgroup)：ratswereusedasthecontrols；(2)Pl(theperiodontitisgroup)：periodontitisgroupwithouttreatment；(3)P2(treatmentgroup)：therats、析mperiodontitisdrinkinghydrogenrichwaterInP1andP2groupssilkligatureswereligatedaroun

6、dthesecondmaxillarymolars；theratswerefedwithsucrosewaterandinoculated、杭thPgingivalisAfterthemodelofperiodontitiswerebuiltupat21thdaytheratsweresacrificed，weobserved：(1)Visualassessmentincludedtoothmobility(TM)，gingivalin

7、dex(GI)，andalveolarboneloss(ABL)(2)Histologicalexamination(3)GSH／GSSGratio(4)theexpressionofOPGandRANKLinperiodontaltissuenecollecteddatawerestatisticallyanalyzedusingvariancetest、析tlltheSPSS100statisticssoftwareResults：

8、TM(p口DJ)，GI(pO01)，ABL(pn卿weresignificantlyreducedinP2compared、)l，ithP1groupLessmonocyteeffusionandboneresorptionlacunaewereobservedinP2groupTheGSH／GSSGratio(p001)andOPGexpression(pa01)increasedinP2group；butRANKLlevelwere

9、notdifferentbetweenthetwogroups，OPG／RANKLratioinP2groupWaselevatedrelativetoP1groupHydrogencouldreducethelevelofoxidativestressandprotecttheperiodontalboneConclusion：Hydrogenmayinfluenceperiodontalinflammatoryresponseand

10、reduceperiodontaltissuedamageandalveolarbonelossinperiodontitisTheeffectmaybethroughreducingperiodontalpartialoxidationlevelandelevatingOPG／RANKLrateTherefore，Hydrogenmaypreventandcuretheinfectiousbonemetabolismdiseasesu