氫飽和水對大鼠實驗性牙周炎抗炎作用的初步觀察.pdf

1、青島大學(xué)碩士學(xué)位論文氫飽和水對大鼠實驗性牙周炎抗炎作用的初步觀察姓名：孟祥嬌申請學(xué)位級別：碩士專業(yè)：口腔基礎(chǔ)醫(yī)學(xué)（牙周病學(xué)）指導(dǎo)教師：鄧婧；袁昌青20120519Abstract川IlllIIIllIIIllllIIIIIY2088140Objective：Chronicperiodontitisisanon—specificinflammationinitiatedbydentalplaquemicroorganismswhichoc

2、curresinperiodontaltissuesModempathogenesisofperiodontitisstudiessuggestthatmostofthetissuedamageisduetothesystemicoverreactiontothepathogensAconsiderablenumberofexperimentsshowthatoxidativestressisanimportantpathogenicm

3、echanisminperiodontitisRecentstudieshavefoundthathydrogenisanovelbiologicalantioxidantswhichhavetherapeuticeffectsonavarietyofdiseasesrelatedtooxidativestress，andhavetheabilitytoslowdownperiodontitisprogressThisStudyobse

4、rvedtheperiodontalinflammationreactioninratsdrinkinghydrogenrichwaterandpreliminarilyinvestigatedtheeffectofhydrogenoninfectiousbonediseaseMethods：24maleSprague—Dawleyrats(8weeksofage)wererandomlydividedinto3groups：(1)N(

5、thenormalgroup)：ratswereusedasthecontrols；(2)Pl(theperiodontitisgroup)：periodontitisgroupwithouttreatment；(3)P2(treatmentgroup)：therats、析mperiodontitisdrinkinghydrogenrichwaterInP1andP2groupssilkligatureswereligatedaroun

6、dthesecondmaxillarymolars；theratswerefedwithsucrosewaterandinoculated、杭thPgingivalisAfterthemodelofperiodontitiswerebuiltupat21thdaytheratsweresacrificed，weobserved：(1)Visualassessmentincludedtoothmobility(TM)，gingivalin

7、dex(GI)，andalveolarboneloss(ABL)(2)Histologicalexamination(3)GSH／GSSGratio(4)theexpressionofOPGandRANKLinperiodontaltissuenecollecteddatawerestatisticallyanalyzedusingvariancetest、析tlltheSPSS100statisticssoftwareResults：

8、TM(p口DJ)，GI(pO01)，ABL(pn卿weresignificantlyreducedinP2compared、)l，ithP1groupLessmonocyteeffusionandboneresorptionlacunaewereobservedinP2groupTheGSH／GSSGratio(p001)andOPGexpression(pa01)increasedinP2group；butRANKLlevelwere

9、notdifferentbetweenthetwogroups，OPG／RANKLratioinP2groupWaselevatedrelativetoP1groupHydrogencouldreducethelevelofoxidativestressandprotecttheperiodontalboneConclusion：Hydrogenmayinfluenceperiodontalinflammatoryresponseand

10、reduceperiodontaltissuedamageandalveolarbonelossinperiodontitisTheeffectmaybethroughreducingperiodontalpartialoxidationlevelandelevatingOPG／RANKLrateTherefore，Hydrogenmaypreventandcuretheinfectiousbonemetabolismdiseasesu