葛根異黃酮對大鼠實驗性牙周炎的治療作用.pdf

1、佳木斯大學(xué)碩士學(xué)位論文葛根異黃酮對大鼠實驗性牙周炎的治療作用姓名：高艷艷申請學(xué)位級別：碩士專業(yè)：口腔臨床醫(yī)學(xué)指導(dǎo)教師：朱建華20080614ABS。l’RACrI’Objective：ThepathogenicmechanismofperiodontaldiseaseisverycomplexandisstillnotwellknownThetBmornecrosisfactoralphparticipateininflammation

2、andhistoclasiaTheanimalexperimentwasdesignedinourstudytoobservetheeffectofthepuerafiaisoflavoneonperiodontitisTheexpressionoftumornecrosisfactoralphinperiodontiumissemiqamntitativelyanalysedtoevaluatethetherapeuticeffica

3、cyofthepuerariaisoflavoneMethods：Partofone—animalexperiment：(1)56Wisterratswererandomlydevidedintosixgroups：(1)Ngroup(normalgroup)：normallyfeeding，noanyspecialhandling；(2)Pgroup(periodontitisgroup)：periodontitisWasinduce

4、dinratsbyplacingapieceofbraidedsilkaroundthecervixofthelowerincisors，andthentheratsweresubjectedtointraperitonealinjectionof05％CMCNasuspensionafteroperationdaily；(3)Plgroup：theratsweresubjectedtointraperitonealinjectiono

5、fpuerariaisoflavone(10mgkg)dailyaftertheratsreceivingtheoperationofperiodontitismodel；(4)P2group：theratsweresubjectedtointmperitonealinjectionofpuerariaisoflavone(20mg|kg)dailyaftertheratsreceivingtheoperationofperiodont

6、itismodel；(5)P3group：theratsweresubjectedtointmpefitonealinjectionofpuemriaisoflavone(40mg／kg)dailyaftertheratsreceivingtheoperationofperiodontitismodel；(6)Tgroup：theratsweresubjectedtointmpefitonealinjectionoftinidazole

7、(17mg／kg)dailyaftertheratsreceivingtheoperationofperiodontitismodelThegingivalindex(GI)wasmeasuredbeforetheratsweresacrificedatendof3weeksForhistopathologicalexaminationthemandibularincisorsandtheperiodontaltisSueswerere

8、movedat3weeksaftertheligatureinductionofperiodontitistoNgroup、Pgroup、P1group、P2group、P3groupnetissueswere凰edin10％neutralbufferedofformaldehydeformorethan48hours，decalcified，embeddedinparaffinandsectionedThesectionswerest

9、ainedwithhematoxylinandeosinItigolo西calchangeswereobservedbymicroscopenleintensityofpositivesignalinperiodontaltissueissemiquantitativlyanalysedbycomputerimageanalysissystemResults：(1)ExperimentalperiodontitisWassuccessf

10、ullyinducedinratsbyplacingapieceofbraidedsilkaroundthecervixofthelowerincisorsatweek4(2)Inligatureinducedperiodontitisgroup，atweek4thebonelossandpositivesignalinperiodontaltissuewerehighersignificantlythanthoseincontrolg