缺氧誘導(dǎo)因子-1α和誘導(dǎo)型一氧化氮合酶在胃癌中的表達(dá)研究.pdf

1、學(xué)校代碼：10132學(xué)員編號：2003107中圖分類號：R7352喲蒙爸逢穹皖i鞘瓣鞋輟MONGO軋l矗黼EDIC焱歉e0乳k蕤GE碩士學(xué)位論文缺氧誘導(dǎo)因子一1儀和誘導(dǎo)型一氧化氮合酶在胃癌中的表達(dá)研究學(xué)科專業(yè)：研究生：指導(dǎo)教師：完成時(shí)閥：病理學(xué)與瘸理生理學(xué)袁宏偉冀文茹教授李超美高級實(shí)驗(yàn)師二0o六年五月oABSTRACllo嗡ectiveHypo)(iaisac(Ⅱ砌onphe㈣enoninthetⅢnornlicroenviroment

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4、hypoxic。Dnditions(1％oXygen)forlh，6h，12h，24handnoHnoX沁吲1djtions，R肼ersetranScription一耐)硼erasechainre8ction(RT—PCR)andimmunocytocherniStrywereusedtodetectmRNA1evelandproteinlevelexprcssk咀o(hù)fHIF—lGandiNoSImmunohistoch鋤istry啪s

5、usedtodetecttheexpressionofHIF～1a，iNoSin45resectedspeciHlenSofgastriccancer8nd10paracarlceroustissueS鼗器ults(1)Semiquantityf汀一PcRs址昭estedthatthee)【一pressionofHIF—lQmRNAinthehypo越cgD叫ps㈣paredwiththenomoxicgroupallhadnotasi

6、gflificantdifference(p005)InnoHnoxicconditiontheexpre蹣knofiNoSn1RNA、^，asataverylowlevelAt1hafterhypo五a，theeXpressionof主NOSmRNAhadnotasig—nificantdifference(pO05)Whi王e6h，12hand24hafterhypoxiatbelevelofexpressionincreasedr

7、apid王y(2)Immunocytocher工1istrysuggestedthatinthenormoxicconditi。ntheexpresShofprotein《HIF—l飪waSn。tdetectedWhiletlep眥einofiNOSwasataverylowlevel。Butbothw黜incrcasingatdifferenthy辯ict婦(3)弧eexpres8ionofHIF一1aandiNOSpmteinwer

8、e667％(30／45)，756％(34／45)ingastriccancerTheexpres—sion《HfF—laproteinweresignificantlycorrelatedwithdifferencia矗on，infil掘牡ion，metaStaSisandTNM(pO。05)Whiletheexpre娥onofiNoSprotein愀s塘Hificantlycor—relatedwithinfiltration，met