轉(zhuǎn)基因抗蟲水稻對田間螟蟲結(jié)構(gòu)的影響及其機(jī)理的初步研究.pdf

1、中國農(nóng)業(yè)科學(xué)院碩士學(xué)位論文轉(zhuǎn)基因抗蟲水稻對田間螟蟲結(jié)構(gòu)的影響及其機(jī)理的初步研究姓名：高玉林申請學(xué)位級別：碩士專業(yè)：農(nóng)業(yè)昆蟲與害蟲防治指導(dǎo)教師：傅強(qiáng)20060601AbstlactEcolo舀caIsafbtyofn鋤sgellicricehasbeenbecommgafocusissue血tlleworldIt’sVeryiIllportantaIldnecessarytoevaluatet11epotentialec010百caIs疵

2、tysystematicallya11dscient訊callybefbretlecommercializationof仃aIlgenicriceInt11isp印er，tllee髓ctof訂ansgenicriceconta誠ng叼7朋c印口to)￡inonfieldcompositionofs廿ipedstemborer(ssB)，(確砌Jq即憎渤脅，arIdpmkstemborer(PSB)，&5冊f婦列備陀瑚were咖died，

3、鋤d沁possiblemechaIlismw越alsopreliminaIystudiedInaddjtion，thesusceptibiI時oft、vostemborerstoBtindividualorcombiIl撕onsw鵲alsostIldied，andthenhelptoguidetheselectionofefI毫ct～etoxinsforbreedingoftransgenicriceThemainresultswere

4、showedasfbllows1TheresuI協(xié)offieldtestsinbothearlyandlacericeindicatedtIatthetransgenlcnceproVidedhighlevelsofcontrolagaillstSSB鋤dPSBThenumberofSSB鋤dPSBlanr∞ontransgenicriceweresi印i矗ca|ltlyreducedcOmparedw汕thenon_仃鋤sgenicc

5、ontr01Amongthestemborerssurvivedont啪蹭enic—cefieId，mepercentageofPsBincre如eds咖incantlywhencomparedwiththatonnon一仃鋤sgeniccon仃D12ThelabOratorybio船sayresultsshowedthatthesuwivaIOfSSBarIdPSBontransgenicricewassiglli矗cantly10w

6、ert11aIlthatonnontran踞eniccontr01ThetoxicactiV時of打ansgenicricetoSSBandPSBdeclinedsign訊cantIyatthelategrowingstagePSBontraIlSgenjc訂cesurvivedobviouslybe釷erthanSSBAsforSSB，nolarvaecouldsurvivetothe5thdayafter_mfestationont

7、舢sgenjcriceAndsomePSBlarvae(2_3~73％)couldsurviveatthe15。dayFurthennore，therewereafewPSBlarvae(16％)couldpupa把a(bǔ)ndsomeofthemcouIdeme唱ence3Toxic時ofsevcralBtprotoxinsa鰣nsttwostemborers(PsBandsSB)wascompa他dbyfeedingneonate1aⅣa

8、eonaIti矗cialdietscontainingBtproto越nsiflthelabOraIolycOnditjonsTheresultsshowedsi舭incamsllSceptibil時di旋rencesbet、veenssBarIdPsBtocrylAb、crylAa、crylBa，withtheexceptionofthecrylcaandcrylAc，PsBwass培nmcalltlylowsusceptibleto

9、thefo盯nerthreeBtproto五ncompafedwithSSBTbevaluatepossjbleiⅡtemctionamongtheseprotoxjn，toxlci可assaywasperfbmledwithmixtIlresof2Btpmtoxinsat1：1ratioAlltIlemixnlreprotoxinshowedsigni矗cantsyne曙istictoxicityactivityagainstssBI

10、ncon訂髂t，onlyc叮1AacrylAbmixtureprotoxinindic砒edmoderatedsyne曙istictoxicityactiv姆againstPsBInmortality—timeresponseexperiments，PsBshowedmoretolerancetoCrylAbandC叫1ActhanSSBwhen訂eatedwjtllloworhighcOncentrationpmtoxjn4111ee

11、懦ctsofthetrallsgenic“ceonovipositiollalpre斯enceofPsB柚dthebiologyof4伊”，P伽加研P陽f珊，aparasitismfofPsB，wereinvest培atedItwaSobservedthatPsBdidnotobviouslyprefem砌tmsgenic—cetothecontrolriceinOvipositionalselection，a11dalso，thetr

12、ansgenicriceshowednosi印incante舵cton也ebiolo百calpar帥derofAg如肌Pmf塒species5Comp刪wjthPSBtreatedwilhthenon_trallsgenicrice，thev耐ationinbjologicaltransofsurvjvedPSBwasnotobservedwhentheywereselected(1～2generation)onn硼踞enicricei

13、nthefield，incIudjngpupalweight，emergencemIe，adultfecundilyandtheLC50toCrylAcTosumupIt’ssuggestedthat協(xié)eV耐edsusceptibil時oft、vostembo塒’totr鋤sgenicricefatherthaIltIleecologicalf她torpresenthere鏟eatlycon蜘butestoabe批funderstall