銀屑病患者T淋巴細胞對表皮c-myc、bcl-2及p53蛋白表達影響的實驗研究.pdf

1、山西醫(yī)科大學(xué)碩士學(xué)位論文銀屑病患者T淋巴細胞對表皮cmyc、bcl2及p53蛋白表達影響的實驗研究姓名：李新華申請學(xué)位級別：碩士專業(yè)：皮膚性病學(xué)指導(dǎo)教師：張開明2002.5.20山疊正科文學(xué)碩士學(xué)t論文InfluenceofPsoriaticTLymphocytesonEpidermalCmyc，lkl2andP53ProteinExpressioninVitro【Abstract】Obiective：Torevealpsoriatic

2、Tlymphocytesactiononepidermalcmyc，bcl2andp53proteinexpressioninordertoinvestigatethemechanismthatpsoriaticTlymphoeytesinduceepidermistoproliferateabnormallyMethods：lmmunohistochemistrymethodwasperformedtodetecttheexpress

3、ionofemyc，bel2andp53inpsoriaticlesions，uninvolvedskinandnormalskinfromhealthycontrolsNormalskinandpsoriaticuninvolvedskinwereincubatedwitllpsoriaticTcellsfor3dayand6daystainedforcmycbel一2andp53expression，incomparisionwit

4、hexpressionbeforecultureandinculturedpsoriaticuninvolvedskin，culturednormalskinandnormalskinculturedwitllnormalTcellsatthesametime，Results：Thereweresignificentoverexperssiunofcmycandp53proteininpsoriaticlesions，butbel一2e

5、xpressionWasrarelyabservedTbeexpressionofpsoriaticuninvolvedskinWasasnomalP53expressioninnormalskinandpsoriaticuninvolvedskinWasstronger瓶erthe3“daycultured誦tllpsoriaticTcellscomparedwiththatbeforecultureandinothercontrol

6、satthesametimeThereweresigniflcentupregulationofcmycexpressionafterthe60daycomparedwitllthatbeforecultureandthe3“dayaftercultureandothercontrolsatthesametime，theexpressionofbcl2wasdecreasedandp53expressionWassimilartotha

7、tculturedafterthe3“dayTherewasnodiffereneebetweennormalskinandpsoriaticuninvolvedskinstimulatedbypsoriaticTcellsrespectivelyConclusions：Cmyc，bcl2andp53proteinexpressionaleabnormalinpsoriaticlesions，whilethoseinpsoriaticu

8、ninvolvedskinarenomalTheresultsshowthattheabnormalexpressionoftheproteinsareveryimportantinpS州aticepidermalhyperplasiaandabnormalpattemofeellulardifferentiationPsoriaticTlymphoeytesCaninfluencecmye，bcl2andp53proteinexpre

9、ssioninnormalskinandpsoriaticuninvolvedskin，whichmightbecentraltoinitiationofpsoriasisPsoriasisisaimmunologicaldiseaseinwhichpathogenicTcellsratherthanepidermalkemtinoeytesareofprimaryimportance[Keywords]PsoriasisTlympho