血紅素氧合酶在妊娠高血壓疾病發(fā)病中的作用.pdf

1、山西醫(yī)科大學(xué)碩士學(xué)位論文血紅素氧合酶在妊娠高血壓疾病發(fā)病中的作用姓名：何玉潔申請學(xué)位級別：碩士專業(yè)：婦產(chǎn)科學(xué)指導(dǎo)教師：成要平20060521西醫(yī)科大學(xué)砸士學(xué)位論文StudiesontheexpressionofhemeoxygenaseinthepathogenesisofhypertensivedisordercomplicatingpregnancyAbstract0bjective：Toexaminetheexpressionof

2、hemeoxygenase—I(HO一11andhemeoxygenase一2(HO一2)inplacentatissueandthelevelofitsbyproductcarbonmonoxide(CO)inperipheralbloodfrompatientswithhypertensivedisordercomplicatingpregnancyandwomenwithnormotentivelatepregnancy，soas

3、toexploretheroleofHO—COsysteminthepathogenesisofhypertensivedisordercomplicatingpregnancyMethods：WeexaminedtheexpressionandlocalizationofHO—1andHO2inplacentatissueofhypertensivedisordercomplicatingpregnancy(studygroup，n=

4、30)andnormotentivelatepregnancy(controlgroup，n=26)usinginsituhybridizationandinamunohistochemistryFurthermore，medical—imagemeasuresystemwasusedforquantitativeanalysisInaddition，thelevelofCO—hemoglobin(COHb)inperipheralbl

5、oodwasstudiedRESULTS：1HO一1andHO一2wereexpressedinplacentatissueofhypertensivedisordercomplicatingpregnancyandnormotentivelatepregnancygroupHO一1isexpressedprominentlyinplacentaltrophoblastcell，HO一2prominentlyintrophoblasta

6、ndvillusvascularendotheliumcell①InsituhybridizationmethoddemonstratedthattheaverageopticaldensityofHO——1mRNAincelloftrophoblastandHO一2mRNAincelloftrophoblastmadvillusvascularendotheliumwere(O260t0032)、r0258O025)and(0252_

7、__00311inhypertensivedisordercomplicatingpregnancygroup，whichhadnosignificantdifferencecomparedwiththoseincontrolgroup(O260O026)、fO2650030)and(026940037)(P005)②Immunohistochemicalmethoddemonstratedthattheaverageopticalde

8、nsityofHO1proteinincelloftrophoblast(O24300341weresignificantlyreducedinplacentafromhypertensivedisordercomplicatingpregnancycomparedwithnorrnotentivelatepregnancygroup(O26600331(P005)TheaverageopticaldensityofHO2protein

9、incelloftrophoblastandvillusvascularendothelium(0240O035)and(02420034)weresignificantlylowerthanthoseincontrolgroupfO261O040)and(0，2670036)(P005)TheexpressionofHO一1andHO2proteininslighthypertensivedisordercomplicatingpre

10、gnancygroupweresignificantlyhigherthanthoseinseverehypertensivedisordercomplicatingpregnancygroup(P005)2ThelevelofCOHb(176％4049％)wassignificantlyreducedinperipheralbloodfromhypertensivedisordercomplicatingpregnancycompar