Development of Strategies for the Constitutive and Tissue Specific Expression of Cry1A(c) Conferring Resistance to Lepidoptera.pdf

1、　　So far, the history oftransgenic crops can be included in two parts. The first era deals with the development of such novel crops. As the time went on, various social, environmental and technical issues related to tran

2、sgenic crops took their birth. The genetic engineering of crop plants is now moving towards the course of correction. Of the agronomic traits improved by using transgenic plants, genetic resistance to pests has attracted

3、 considerable attention. Now a days, a number of approaches to pest control via genetic engineering have been developed and genetically engineered crops expressed insecticidal characteristics. he transformation experimen

4、ts to have an efficiency comparison with the former two constructs. The major results in the Part-Ⅱ of dissertation can be summarized as below;An attempt was made to optimize conditions for an efficient embryogenic callu

5、s induction and plant regeneration system from mature seeds of japonica and indica rice (Oryza sativa L.) cultivars, We have compared the expression level of Cauliflower Mosaic Virus (CaMV) 35S promoter and the Rice Suc

6、rose Synthase-1 promoter (RSuS-1), when fused to a common reporter gene gusA. The chimeric genes were introduced into a japonica rice Xiushui-11cultivar Gus specific activity generated by the promoters was quantified usi

7、ng fluorometric method in the vegetative tissues such as leaves, stem, roots, seed coat and seed endosperm+emrbryo. The results demonstrated that the expression level produced by CaMV 35Spromoter was roughly tenfold an

8、d twofold higher than RSuS-1 and RSuS-2 in the leaves, roots, seed coat and stem, respectively. Gus specific expression by RSuS-1 and RSuS-2 in the seed endosperm+embryo was negligible. The overall activity of CaMV 35S

9、 in the whole plant vegetative tissues was still three fold greater. A novel methodology for the GUS staining of whole rice plant tissues was also reported. The histochemical activity generated by RSuS-1 was detected i