酶促合成頭孢羥氨芐以及酶的固定化研究

1、浙江大學理學院碩士學位論文酶促合成頭孢羥氨芐以及酶的固定化研究姓名：杜莉莉申請學位級別：碩士專業(yè)：化學指導教師：吳起20100101摘要AbstractBecauseofitssimilarityinstructure，cefadroxilpossessesmanyofthesamecharacteristicsaScephalexinandcephradineThemostapparentadvantagesofcefadroxilo

2、verthereferencecompounds，however，lieinitspharmacokineticpropertiesAndcefadroxilhasbeenshowntohavealongerserumhalflifeafteroraldosagetohumans，aslowerurinaryexcretionrate，andagreaterareathaneithercephalexinorcephradineInad

3、dition，itwasalsonotedthattheapparentvolumeofdistributionofcefadroxilisgreaterthanthatofcephalexinBecauseofcefadroxil’Sbroadrangeofantimicrobialactivityanditsfavorablepharmacokineticprofileinman，synthesisthisantibioticina

4、greenwayarecurrentlyunderstudybyUSThisthesisfocusesontheenzymaticsynthesisofcefadroxilinfullyorganicmediumWealsesearchedtheaqueousbuffer／dichloromethanetwo—phasesystemandimmobilizationofpenicillinGacylaseonthefunctionali

5、zedSepharoseCL6BTheefficientenzymaticsynthesisofcefadroxilinfullyorganicmediumwassuccessfullyachievedwithDPhydroxyphenylglycinemethylester(DHPGM)astheactivatedacyldonorAseriesoforganicsolventswithdifferentpolaritywerescr

6、eened，amongwhichdichloromethanewasthebestTheinfluencefactorsincludingtheenzymeloading，reactiontemperature，reactiontime，initialsubstrateconcentrationandmolarratioofsubstratesontheenzymaticsynthesisofcefadroxilinpuredichlo

7、romethaneweresystematicallyexaminedAsaresulthighyield(47％)andS／Hratio(045)weresuccessfullyachievedwith100mmol／L7ADCAand200mmol／LD—HPGMundertheoptimumconditionsMediacomposedwithdifferentorganicsolventswerescreenedandaqueo

8、usbuffer／dichloromethanetwo—phasesystem(40／60，v／v)wasfoundtobeabletemarkablyimprovetheenzymeactivityTheconversionof7ADCAandDHPGMwasgreatlyimprovedAfter24hreactiontheconversionof7ADCAwas598％andS／Hratioof06wereachievedinre