26761.福氏2a志賀氏菌2457t的cobb，gtrⅱ和ycid基因功能的研究

1、南昌大學(xué)碩士學(xué)位論文福氏2a志賀氏菌2457T的cobB，gtrⅡ和yciD基因功能的研究姓名：劉小青申請學(xué)位級別：碩士專業(yè)：理學(xué)指導(dǎo)教師：魏華王恒樑20100608AbstractABS眥CTGlucosyltmnsferases(Gtrs)and0一aeetyltmnsferase(Oae)belongtointegralmembraneproteins，and也eyareresponsibleforthedifferentiati

2、onofserotypesofShigellasppGtrlIisaspecificserotype，whichcanglucosylatethe0antigenrepeatunitinrhamnoseIII，andwasprovedtobeusefulindiscriminatingserotypeYandserotype2aInprokaryoticorganisms，CobbdeaeetylatesacetylCoAsynthet

3、ase(Acs)AsthehomologofeucaryotaSir2(Silentinformationregulator2)，theCobBWasdeducedtoberelatedwithchromatinsilencingYciDisahypotheticalproteinbelongingtotheOmpWfamilyThevirulentproteinexpressionofShigellaflexneriiscontrol

4、ledbytemperatureieexpressingat370Corlosingexpressionbelow300CInourpreviousstudy，thecomparativeproteomicresultsofSflexneri2a2457Tculturedat370Cand300CshowedthattheYciDexpressionWasupregulatedat370C，whichinspiredUSthatthey

5、ciDmightbeavirulenceassociatedgeneToinvestigatethethreegenes’functionsandmakeitclearwhethertheyareassociated謝mvirulence，thecobb，gtr／／hadbeensuccessfullyknockedoutbyusinganimprovedkRedrecombinationsystemThemutantof聲國isast

6、rainconstructedinOurpreviousstudyThesemutantsaretermedasAcobB：：kan，AgtrII：：kanAyciD：：kanTheresultsofscrenytestsshowedthatAcobB：：kan，AgtrlI：：kancausedaslighterinflammationthanthewildstrainandtheAyciD：：kanistheslightestone

7、Subsequentlythewholecellproteinsofwildstrainandthesethreemutantswerepreparedandcomparedbyusingtwo—dimensiongelelectrophoresis(2一DE)，thecomparativeproteomicsresultsshowedthatAcobB：：kanagtrn：：kanpossessedlittledifferencesw

8、iththewildstrain；howeveradownregulatedproteinWasobservedinAyciD：：kanAfultherresearchonAyciD：：kanfunctionsWascarriedoutandthewholeoutermembraneproteinsofmutantandwildstrainwerepreparedanddetectedby2DE，3downregulatedouterm

9、embraneproteinswereobservedDuringvirulentevaluationthecompetitionindex(cI)ofinfectedHelais08andthemurinelunginfectedis09，Thus，wec趾concludethatcobbandgtr刀mightnotaffecttheinvasions。flezneritheabsenceofyciDdecreasedthesfle