45497.磁共振技術(shù)及其在絲素蛋白結(jié)構(gòu)與功能研究中的應(yīng)用

1、V9523039欠擘碩士學(xué)位論文學(xué)校代碼：10246學(xué)號(hào)：032044005磁共振技術(shù)及其在絲素蛋白結(jié)構(gòu)與功能研究中的應(yīng)用院系：高分子科學(xué)系專業(yè)：高分子化學(xué)與物理姓名：胡炳文指導(dǎo)教師：周平教授完成日期：2006年6月●●●●●復(fù)旦大學(xué)碩士論文磁共振技術(shù)及其在絲素蛋白結(jié)構(gòu)與功能研究中的應(yīng)用Abstract1InvestigatetheeffectofCu(11)ontheconformationtransitionsofsilkfibro

2、inwithEPRspectroscopyHerewedevelopasimulationapproachbasedonthesecondorderperturbationtheorytosimulatethesilkfibroinCu(II)complexes’theEPRspectrawithhyperfinecouplingsandmultiplecoordinationcomponents，andefficientlyrevea

3、lthefunctionofCu(II)inthespinningprocessofsilkworm2InvestigatetheeffectoftemperatureontheconformationtransitionsofsilkfibroinobtainedfromthesilkglandwithNMRspectroscopyWefoundoutthatsilkfibminintheanteriorpartofsilkgland

4、isinclinedtocrystallizewhilethatintheposteriorpartremainstheconformationofrandomcoilFurtherworkneededtobedone3Investigatethefunctionandthestructureofthespidersilk—likematerialswhichissynthesizedbypolypeptide(AIa)nandapol

5、ymerwithNMRspectroscopyandDFT(DensityFunctionTheory)calculationWefoundthatbothpolystyreneandpolyisopreneexertedverylittleeffectonthe(AIn)ninthespidersilklikematerialsThepropertyofthespidersilklikematerialsdependsonthepro

6、pertyofthepolymer4Applicationofthcgeneralized2DcorrelationanalysisapproachtothefieldofNMR111isworkcompfiSeSoftwopartsFirstlyweapply2DcorrelationanalysisapproachtoanalyzethepHperturbedNMRspectraandcreate2Dheterospectralco

7、rrelationspectrabetweenNMRandRamanspectraofsilkfibroin，effectivelycontributingtotheassignmentsofthecomplicateNMRandRamanspectraSecondlyweapply2DcorrelationanalysisapproachtoanalyzethetraditionalFouriertransformation2Dcor

8、relationNMR(FT2DNMR)spectraThetraditionalFT2DNMRexperimentsneedtoacquireenoughFID(FreeInductionDecay)inthesecondtimedomainiIlordertoperformFouriertransformation1eadingtolongexperimenttimeWedevelopanewapproachGenemlized2D