28896.百合屬部分種親緣關系與岷江百合群體遺傳結構研究

1、南京林業(yè)大學博士學位論文百合屬部分種親緣關系與岷江百合群體遺傳結構研究姓名：吳祝華申請學位級別：博士專業(yè)：林木遺傳育種指導教師：施季森20080701ResearchonGeneticRelationshipConservationGeneticsofLiliumSpeciesandofLregaleAbstractChinaisnaturedistributionalcenterofLiliumspecies，Researcheson

2、wildresourcesareanimportantbasetobreedneweultivars(1)Thedistribution，ecologyenvironmentof19Liliumspeciesand2unknowntypesinmostofSouthwardofChinaandnortheastofChinawereinvestigatedandcollectedLbrowniivarviridulum，三1ancifo

3、lium三tsingtauenseandLspeciosumvargloriosoides，Ldauricum，LconcolorLdistichum，Lpumilum，L。browniivarviridulum，LbrowniiL1ancifolium三1eucanthum，Lbrownii，Lbrowniivarviridulum，￡regale，工sargentiae，￡slllphurreum。LhenryiLdavidiiLr

4、osthorniiLduchartreiL10phophorumand三sp1，三sp2wereinvestigatedandcollected(2)Geneticrelationshipwasanalyzedbasedonthedataofpollenmorphology，ISSRmolecularmarker，ITSsequencesofnrDNAandtrnLFsequencesofcpDNAfromeachspeciesTher

5、esultshowthattherewerecloselyrelationshipamongLrosthornii，L1ancifolium，三sp2，三davidii，Lconcolor；amongLbrowniivarviridulum，￡brownii，Lsp1；betweenLTsingtauenseandLdistichumandamong三dauricum，Lsulphureum，L1eucanthum，LtogaIeLsa

6、rgentiae(3)ThegeneticdiversityandspatialautocorrelationofgeneticstructureofLregalewasanalyzedbaseonISSRmolecularmarkerAtthespecieslevel，theproportionofpolymorphiclociwas977％，theeffectivenumberofallele(Ne)was19944，Shannon

7、diversityindex(I)was03339Atthepopulationlevel，theaverageexpectedheterozygosiswas0664Shannondiversityindex(I)was02720Theyshowedthatgeneticdiversityof三regalewashighTheGstof三regalewas016l9，(1isp一坳叩)／Hspwas01537，theresultbyA

8、MOVAanalysisindicatedthatthevariationwithinpopulationaccount838％andvariationamongpopulationsaccountedforl1849％，andthegeneflOWwas2588TheydemonstratedthattherelationshipofpopulationswascloserGenedifirerentiationwasacutewit

9、hinpopulationThespatialautoeorrelationofgeneticstructureof4populationswereanalyzedTheMoran’S1eorrelogramrevealednosignificantspatialstructureinthe4populationsItindicatedthatgeneticvariationsofthemostpolymorphiclociinthes

10、epopulationswererandomlydistributedHowever，thereweregapsatthedistanceof3～4m，5～6and8～10m，andtherewereintrusionatalittlepolymorphiclociKeywords：Lilium；Geneticrelationship；Pollenmorphologycharacters；ISSRmolecularmarker；ITSs