31367.不同潤濕性sicoh膜層的血液相容性和內(nèi)皮細(xì)胞粘附性的研究

1、重慶大學(xué)碩士學(xué)位論文不同潤濕性SiCOH膜層的血液相容性和內(nèi)皮細(xì)胞粘附性的研究姓名：張勤申請學(xué)位級別：碩士專業(yè)：生物物理學(xué)指導(dǎo)教師：王貴學(xué)20090605重慶人學(xué)碩二學(xué)位論文英文摘要ABSTRACTThelowtemperatureplasmadepositiontechniquewasusedinthisstudytoprepareSiCOHcoatingswithdifferentwettabilityThesurfaceprope

2、rtiesofSiCOHcoatingswereexaminedbyFTIRATRandcontactanglemeasurementsystemTheblood—compatibilityandcellaffinityofSiCOHcoatingsinvitroWasdetectedinthisstudyanditfocusedonstudyingtheeffectofwettabilityonvisiblebloodcomposit

3、ionandHUVECs，includingbloodplateletsadhesion，deformationandaggregation；plasmaproteinadsorption；bloodcoagulationfactorsactivation；HUVECsadhesionandgrowthThisstudyaimedtofindthecorrespondingtechnicsofplasmacoatingwithexcel

4、lentbloodcompatibilityandcellaffinityTheresultsofwettabilityshowedthat，withincreaseofflowratioof02(from0sccmto5sccrn)thewatercontactanglesdecreasefrom988300to27821oTheresultsofFTIRATRalsoshowedthathydrophobicgroupsinSiCO

5、Hcoating，suchasSiH、CH3、SiOSiandCCwouldbereplacedbyhydrophilicgroups(OH、CO)withincreaseofflowratioof02Withincreaseofhydrophilicity，theadsorbentamountsoffibrinogen(Fib)onSiCOHcoatingdecrease，however，theadsorbentamountsofal

6、bumin(Alb)havenosignificantdifferencebetweenallgroupsTheresultsdemonstratethatFibadsorptionwouldmoredependonhydrophilicityofsurfacecomparewithAlbTheexperimentsofactivatedpartialthromboplastintime(aPTT)showedthattheaPPTof

7、sampleais24816s，itWasshortestinallsamples，Samplesd，eandfwereneartonormalvalueTheresultsofaPTTindicatedhydrophobicSiCOHcoatinghavesignificantstimulationforintrinsiccoagulationsystemTheresultsofbloodplateletsexperimentsals

8、oapprovedthathydrophilicSiOHcoatingcouldreduceplateletsadhesion，deformationandaggregationHoweverthenumbersofHUVECsaremostwhensurfaceofwatercontactanglesarebetween441340and335650itilluminedthatHUVECswouldadhereandspreadon

9、thesurfaceofmaterialswithbalanceofhydrophilicityandhydrophobicityConsideringallresultsinthisstudy，wecouldgetaconclusionthatthebloodcompatibilitywouldbeimprovedwithsurfacehydrophilicityhowevercelladhesionwoulddependonsurf