用基因標(biāo)記法研究紫色土上施鐵鉬對花生根瘤菌有效性及競爭性的影響.pdf

1、四川農(nóng)業(yè)大學(xué)碩士學(xué)位論文用基因標(biāo)記法研究紫色土上施鐵鉬對花生根瘤菌有效性及競爭性的影響姓名：于景麗申請學(xué)位級別：碩士專業(yè)：土壤學(xué)指導(dǎo)教師：張小平李登煜20030501AbstractInthisresearch，twotypesofsoilwhichwerelackofironandmolybdenumwereusedinpotexperimentThreestrainsofBradyrhizobiumSpr37，Spr45，Spr3—

2、5andtheircorrespondinggenemarkerstrainsgusA3—7gusA45，gusA35，celB3—7，celB45celB35withg“鰣andcelBgenewereselectedtoinoculateTianFu9peanutRootnoduleformedbygenemarkerstrainshadtheabilitytoproducespecialcolorwhenreactingwithd

3、etectingreagentWeusedthischaracteristictodetecttheeffectofapplyingironandmolybdenumanatheirdifferentlevelofironandmolybdenumonthesymbioticeffectivenessandcompetitivenessofpeanutBradyrhizobiumstrainsResultsshowedthatincal

4、careouspurplesoilbecauseofavailableirondeficient，sprayingiron，inoculatation，sprayingironinoculationcouldallpromotesymbioticnitiogen—fixtioneffectofpeanut—Bradyrhizobium，theireffectfromhighertolowerweresprayingironinocula

5、tioninoculatationironSpraying02％FeS04H20solutioncouldincreaseshootdryweight，noduleweight，totalnitrogencontent，totalchlorophyllcontent。muchmorethan03％FeS04H20solutionCorrelationcoefficientbetweentotalchlorophyllandtotalni

6、trogen，peanutyields，shootdryweight，noduleweightwererespectivelyO806、0637、0795、O585Spraying02％FeS04H20solutioncouldpromotecompetitiveabilityofpeanutandinoculatedBradyrhizobiumstrainsbetterthanthatofO3％theircompetivenessin

7、tomwasSpr45Spr37Sp35Ratioofoccupancydetectedbytwogenemarkertechniqueshadnosignificantdifference，averagenumericalvalueofthemwa$respectively7946％，7562％744l％Inacidpurplesoilbecauseofavailablemolybdenumdeficienteffectofapply

8、ingMoinoculationWaSbetterthaninoculation，andinoculationwasbeRerthanapplyingMoEffectofO4％NH4M003xH20solutionwasbetterthanO2％whentheywereappliedalone，whileO2％NH4M003xH20solutionWasbetterthanO4％ininoculatingBradyrhizobiumst